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  • 1
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    Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H. cumingii.
    Description: Published
    Keywords: Triangle mussel ; Hyriopsis cumingii ; Microsatellite ; Polymorphism ; Genetic diversity ; Isolation ; Microsatellite
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.631-640
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  • 2
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    Isolation and characterization of 13 new microsatellite markers in the triangle mussel (Hyriopsis cumingii) (2021)
    In:  http://aquaticcommons.org/id/eprint/22468 | 18721 | 2018-04-10 22:07:07 | 22468 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-06
    Description: Microsatellite enriched library of Hyriopsis cumingii was constructed according to the strong affinity between biotin and streptavidin. One hundred clones with foreign inserts were sequenced and 65 clones were screened for usable microsatellites, of which 36 were deemed unique, of sufficient length (more than 8 repeats) and possessed adequate flanking regions for primer designment. Among 36 primer pairs designed, 25 yielded scorable amplification products. Upon testing 30 individuals were sampled using the 25 pair primers from Dongting Lake of Hunan Province, China. Then thirteen polymorphic microsatellite markers were isolated and characterized. These loci exhibited high levels of genetic polymorphism, so the observed number of alleles per locus ranged from 4 to 9. The ranges of observed and expected heterozygosity were 0.2543 to 0.8913 and 0.3629 to 0.8217, respectively, and the average polymorphic information content was 0.5198. Two microsatellite loci were significantly deviated from Hardy-Weinberg equilibrium due to the presence of null alleles, and no linkage disequilibrium found. These microsatellite loci will be useful for assessing the genetic diversity and population structure of H. cumingii.
    Keywords: Aquaculture ; Biology ; Hyriopsis cumingii ; Microsatellite ; Polymorphism ; Genetic diversity ; China
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 631-640
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of the sheet rigidity on strength of the spot weld tension shear joint (1990)
    Springer
    Acta mechanica solida Sinica 3 (1990), S. 403-416 
    Details
    ISSN: 0894-9166
    Keywords: spot welded joint ; stress intensity factor ; fatigue life ; tension test ; fatigue test ; finite element method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract This paper presents some test and analysis results for a spot welded joint subjected to tensile and alternate load. The effect of sheet rigidity on the tensile strength and fatigue life of the spot welded joint is studied by using the stress intensity factorsK I,K II,K III and an “effective stress intensity factor” Kϕθmax calculated by the finite element method for crack around the nugget. The results show that the “effective stress intensity factor” Kϕθmax is an essential parameter for estimating the fatigue life of the spot welded joint.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02284772
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Hepatitis B virus core protein interacts with the C-terminal region of actin-binding protein (2000)
    Springer
    Journal of biomedical science 7 (2000), S. 160-168 
    Details
    ISSN: 1423-0127
    Keywords: Hepatitis B virus ; HBV core protein ; HBV precore protein ; Actin-binding protein ; Chronic HBV infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Hepatitis B viral core protein is present in the nucleus and cytoplasm of infected hepatocytes. There is a strong correlation between the intrahepatic distribution of core protein and the viral replication state and disease activity in patients with chronic hepatitis. To understand the role of core protein in the pathogenesis of HBV, we used a yeast two-hybrid system to search for cellular proteins interacting with the carboxyl terminus of core protein, as this region is involved in a number of important functions in the viral replication cycle including RNA packaging and DNA synthesis. A cDNA encoding the extreme C-terminal region of human actin-binding protein, ABP-276/278, was identified. This interaction was further confirmed both in vitro and in vivo. In addition, the extreme C-terminal region of ABP-276/278 interacted with the nearly full-length HBV core protein. Since this region is present in both the core and the precore proteins, it is likely that both core and precore proteins of HBV can interact with the C-terminal region of ABP-276/278. The minimal region of ABP-276/278 which interacted with the HBV core protein was the C-terminal 199 amino acid residues which correspond to part of the 23rd repeat, the entire 24th repeat and the intervening hinge II region in ABPs. The potential functional outcome of ABP interaction in HBV replication and its contribution to the pathological changes seen in patients with chronic HBV infection are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02256623
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  • 5
    Electronic Resource
    Electronic Resource
    The solution of a crack emanating from an arbitrary hole by boundary collocation method (1990)
    Springer
    Applied mathematics and mechanics 11 (1990), S. 669-678 
    Details
    ISSN: 1573-2754
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Mathematics , Physics
    Notes: Abstract In this paper a group of stress functions has been proposed for the calculation of a crack emanating from a hole with different shape (including circular, elliptical, rectangular, or rhombic hole) by boundary collocation method. The calculation results show that they coincide very well with the existing solutions by other methods for a circular or elliptical hole with a crack in an infinite plate. At the smae time, a series of results for different holes in a finite plate has also been obtained in this paper. The proposed functions and calculation procedure can be used for a plate of a crack emanating from an arbitrary hole.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02017482
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  • 6
    Electronic Resource
    Electronic Resource
    Stress intensity factors of a plate with two cracks emanating from an arbitrary hole (1990)
    Springer
    Applied mathematics and mechanics 11 (1990), S. 723-732 
    Details
    ISSN: 1573-2754
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Mathematics , Physics
    Notes: Abstract In this paper, Muskhelishvili complex function theory and boundary collocation method are used to calculate the stress intensity factors (SIF) of a plate with two cracks emanating from an arbitrary hole. The calculated examples include a circular, elliptical, rectangular, or rhombic hole in a plate. The principle and procedure by the method is not only rather simple, but also has good accuracy. The SIF values calculated compare very favorably with the existing solutions. At the same time,the method can be used for different finite plate with two cracks emanating from a hole with more complex geometrical and loading conditions. It is an effective unified approach for this kind of fracture problems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02015146
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  • 7
    Electronic Resource
    Electronic Resource
    1H and 13C NMR study of the adsorption of alcohols on charcoals with various pore structuresProject supported by National Natural Science Foundation of China. (1989)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 27 (1989), S. 987-991 
    Details
    ISSN: 0749-1581
    Keywords: Adsorption NMR ; Charcoal ; Alcohols ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A 1H NMR study of methyl, ethyl, n-propyl, isopropyl and allyl alcohols adsorbed on charcoals with various pore structures showed that the adsorption mechanism is by capillary condensation in pores with radii smaller than 30 Å, and by mono-/multi-layer adsorption on the solid surface of pores with larger radii. This mechanism is identical with that of adsorbed hydrocarbons. The 13C NMR parameters suggest that the adsorbed alcohols are preferentially oriented in the monolayer, with the methyl groups bonded to the solid surface. Longer chain alcohols are bent in capillaries with radii smaller than 10 Å. The broadening of the 13C resonances was partly eliminated in magic angle spinning experiments by using a spinning rate of 1.5-1.8 kHz.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260271015
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  • 8
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    Study of Quaternary activities of the Red River Fault (I) (1984)
    In:  J. Seism. Res., Tokyo, Am. Geophys. Union, vol. 7, no. 5946, pp. 39-51, pp. 8043
    Details
    Publication Date: 1984
    Keywords: Geol. aspects ; Earthquake precursor: prediction research ; China ; Fault zone ; part1 ; JSR
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    BIBLIOGRAPHY SEISMOLOGY
  • 9
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    Red River and associated faults, Yunnan Province, China: Quaternary geology, slip rates, and seismic hazard (1984)
    In:  Geol. Soc. Am. Bull., Tokyo, Am. Geophys. Union, vol. 95, no. 5946, pp. 686-700, pp. 8043
    Details
    Publication Date: 1984
    Keywords: Fault zone ; Geol. aspects ; Earthquake hazard
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    BIBLIOGRAPHY SEISMOLOGY
  • 10
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    Study of Quaternary activities of the Red River Fault (II) (1984)
    In:  J. Seism. Res., Tokyo, Am. Geophys. Union, vol. 7, no. 5946, pp. 171-185, pp. 8043
    Details
    Publication Date: 1984
    Keywords: Geol. aspects ; Earthquake precursor: prediction research ; China ; Fault zone ; part2 ; JSR
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    BIBLIOGRAPHY SEISMOLOGY
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