ISSN:
1432-0827
Keywords:
Key words: Estrogen receptor — Osteoblastic cell — TSC-36 — Follistatin-related polypeptide.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Abstract. Using an osteoblastic cell line (CDO7F) that was selected as an estrogen receptor positive cell by immunocytochemical detection and reverse transcription–polymerase chain reaction (RT–PCR) subtractive cDNA cloning using oligo(dT)30-Latex and PCR was performed to isolate a gene that is induced by estrogen. A cDNA clone was isolated and its nucleotide sequence identified it as TSC-36, previously called follistatin-related polypeptide. Northern blot analysis showed that the TSC-36 gene expression was certainly upregulated by estrogen. Tamoxifen also upregulated this gene expression. These findings indicate that TSC-36 may be one of the estrogen-regulated genes in osteoblastic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002239900355
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