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  • 1
    Electronic Resource
    Electronic Resource
    Zygotic induction of plasmid ssb and psiB genes following conjugative transfer of Incl1 plasmid Collb-P9 (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Incl1 conjugative plasmid Collb-P9 carries a psiB gene that prevents induction of the SOS response in host bacteria. This locus is located 2.5 kb downstream of the ssb (single-stranded DNA-binding protein) gene in the leading region. This portion of Collb is strikingly similar to part of the leading region of the otherwise distinct F plasmid. Expression of psiB and ssb is increased when the host cell is exposed to an SOS-inducing treatment or the Collb transfer system is derepressed. Moreover, expression of both genes on a derepressed plasmid is strongly enhanced in conjugatively infected recipient cells. Carriage of the psiB gene by Collb is shown to prevent a low level of SOS induction following conjugation. Plasmid ssb and psiB genes may function to promote installation of the replicon in the new cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01507.x
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  • 2
    Electronic Resource
    Electronic Resource
    DNA-independent transport of plasmid primase protein between bacteria by the I1 conjugation system (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 38 (2000), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The ColIb-P9 (IncI1)-encoded conjugation system supports transfer of the plasmid T-strand plus hundreds of molecules of the Sog polypeptides determined by the plasmid primase gene. Here, we report that Sog primase is abundantly donated to the recipient cell from cells carrying a non-transferable ColIb plasmid deleted of the nic site essential for DNA export. Such DNA-independent secretion of Sog primase is typical of authentic conjugation, both in being blocked when the recipient cell specifies the entry exclusion function of ColIb and in requiring the thin I1 pilus encoded by the ColIb pil system under the mating conditions used. It is proposed that Sog polypeptides form a complex with the ColIb T-strand during conjugation and aid DNA transport through processive secretion of the proteins into the recipient cell. Functional and genetic relationships between the ColIb conjugation system and other type IV secretion pathways are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2000.02164.x
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  • 3
    Electronic Resource
    Electronic Resource
    Evasion of type I and type II DNA restriction systems by Incl1 plasmid Collb-P9 during transfer by bacterial conjugation (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Transmission of unmodified plasmid Collb-P9 by bacterial conjugation is markedly resistant to restriction compared with transfer by transformation. One process allowing evasion of type I and II restriction systems involves conjugative transfer of multiple copies of the plasmid. A more specialized evasion mechanism requires the Ard (alleviation of restriction of DNA) system encoded by Collb. The ard gene is transferred early in conjugation and specifically alleviates DNA restriction by all known families of type I enzyme, including Eco K. Collb has no effect on EcoK modification but this activity is impaired by multicopy recombinant plasmids supporting overexpression of ard. Genetic evidence shows that Ard protects Collb from Eco K restriction following conjugative transfer and that this protection requires expression of the gene on the immigrant plasmid. It is proposed that carriage of ard facilitates transfer of Collb between its natural enterobacterial hosts and that the route of DNA entry is important to the restriction-evasion mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01366.x
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  • 4
    Electronic Resource
    Electronic Resource
    Transient transcriptional activation of the IncI1 plasmid anti-restriction gene (ardA) and SOS inhibition gene (psiB) early in conjugating recipient bacteria (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 31 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The ardA gene of the enterobacterial plasmid ColIbP-9 acts to alleviate restriction of DNA by type I systems, while psiB inhibits induction of the bacterial SOS response. Both genes are transferred early in a round of bacterial conjugation as part of the plasmid leading region. We report here that ardA and psiB are transcribed transiently after their conjugative transport into the recipient cell. Transcript levels, monitored by competitive reverse transcription–polymerase chain reaction (RT–PCR) amplification of RNA templates, started to increase about 5 min after the initiation of conjugation in a cell population and probably before the first round of plasmid transfer was completed. Genetic evidence is given that the expression of ardA and psiB is activated when the genes enter the recipient cell on the transferring plasmid strand. It is proposed that these and other leading region genes function to promote the establishment of the immigrant plasmid in the new host and are expressed by transcription from promoters active only in single-stranded DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01153.x
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  • 5
    Electronic Resource
    Electronic Resource
    A plasmid DNA primase active in discontinuous bacterial DNA replication (1981)
    [s.l.] : Nature Publishing Group
    Nature 290 (1981), S. 217-221 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A DNA primase encoded by an IncIα plasmid promotes efficient DNA replication in a primase-defective mutant of Escherichia coli. This finding implies that the plasmid enzyme can prime discontinuous DNA synthesis of the bacterial chromosome. The plasmid gene encodes two large, antigenically ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/290217a0
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  • 6
    Electronic Resource
    Electronic Resource
    Induction of prophage λ during the division cycle of Escherichia coli (1975)
    Springer
    Molecular genetics and genomics 139 (1975), S. 245-254 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When synchronous populations of Escherichia coli B/r (λ) were exposed to low doses of ultraviolet light, the yield of infective centres varied with cell age. The yield was highest if the lysogenic bacteria were irradiated at a time which coincides approximately with the termination of rounds of DNA replication and it was lowest when dividing cells were irradiated. No such variation was detected following either irradiation of excision-defective lysogenic cells or thermal induction of λcI857 prophage in irradiated bacteria. It is suggested that the variation reflects a relationship between prophage induction and inhibition of cell division. This hypothesis is supported by data showing that irradiation promoted induction and curtailed division in E. coli K12 dnaA mutants which were dividing in the absence of DNA replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268975
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  • 7
    Electronic Resource
    Electronic Resource
    The kinetics of bacterial DNA synthesis during indirect induction of prophage λ (1972)
    Springer
    Molecular genetics and genomics 119 (1972), S. 49-55 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The possibility that the processes of direct and indirect induction of prophage λ can be unified on the basis of a temporary inhibition of bacterial DNA synthesis has been examined. The kinetics of DNA synthesis in non-lysogenic bacteria were not significantly affected during a 60 min mating with UV-irradiated ColI drd donor cells. In contrast, direct irradiation of the bacteria with a UV dose which induced phage development with an efficiency similar to that obtained by indirect induction caused an abrupt cessation of DNA synthesis lasting for about 12 min. It is concluded that indirect induction of prophage λ differs from most treatments which induce directly in that it is not associated with an inhibition of host DNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270443
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  • 8
    Electronic Resource
    Electronic Resource
    Conjugational synthesis of F lac + and Col I DNA in the presence of Rifampicin and in Escherichia coli K12 mutants defective in DNA synthesis (1974)
    Springer
    Molecular genetics and genomics 134 (1974), S. 143-156 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When F lac + donors were mixed with irradiated recipient bacteria, there was a stimulation of DNA synthesis in the recipients equivalent to the amount of DNA transferred. Rifampicin had little effect on such conjugational synthesis of F′ and ColI DNA during the first 30 min of mating. In matings of temperature-sensitive (ts) dnaB70 mutants at 43° C, transfer of F lac + and ColI DNA was associated with an equal amount of conjugational DNA synthesis in the two parents, the amounts being unaffected by the polA1 mutation. Rifampicin abolished conjugational DNA synthesis in dnaB recipients mated with rifampicinresistant F lac + donors but it stimulated synthesis in equivalent matings involving ColI. Although F lac + and ColI DNA was transferred to (ts) dnaE486 mutants at 43° C, transfer was not associated with either enhanced DNA synthesis in the recipients or the extensive synthesis in them of DNA which was convertible into covalently closed circular molecules by brief incubation at a permissive temperature. These results imply that DNA polymerase III is essential for synthesis of the strands complementary to the transferred F- and I-like plasmid DNA. In the case of ColI, the process is resistant to rifampicin both in the presence and absence of dnaB function whereas for F lac +, it is sensitive when dnaB gene product is thermally inactivated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268416
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  • 9
    Electronic Resource
    Electronic Resource
    A novel priming system for conjugal synthesis of an IncIα plasmid in recipients (1979)
    Springer
    Molecular genetics and genomics 175 (1979), S. 275-279 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Synthesis of DNA complementary to the transferred strand of an IncIα plasmid has been shown previously to require DNA polymerase III. The possible involvement of the two defined priming proteins of Escherichia coli K12, RNA polymerase and primase, in initiating this conjugal DNA synthesis has been examined. Primase was inactivated using temperature-sensitive dnaG3 mutants and RNA polymerase was inhibited using rifampicin. When these two proteins were simultaneously inactivated in both parental strains, the average recipient synthesised at least one single-stranded equivalent of R144drd-3 before the rifampicin-treated donors lost the ability to transmit DNA. It is proposed that the product of a plasmid transfer gene is responsible for initiating this DNA synthesis in recipients. The results imply that this protein is supplied by the donors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397227
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  • 10
    Electronic Resource
    Electronic Resource
    Conjugative transfer of IncI1 plasmid DNA primase (1984)
    Springer
    Molecular genetics and genomics 197 (1984), S. 461-466 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary DNA primase of Collb-P9drd-1 generates RNA primers that are thought to initiate DNA synthesis on the conjugatively transferred strand of the plasmid. To examine whether plasmid-specified primase is transferred during conjugation, we exploited the property of the enzyme to promote bacterial DNA replication in dnaG (primase-defective) mutants of Escherichia coli. It was found that dnaG3 recipient cells, treated with rifampicin to inhibit transcription, recovered ability to synthesise bacterial DNA by a process requiring an active plasmid primase gene in donor cells and a functional conjugation system. A non-transferable primase gene in the donor strain complemented a primase-negative derivative of ColIb-P9drd-1, confirming that the enzyme responsible for recovery was supplied by donor cells. The implication is that certain proteins are transmitted from donor cells to promote conjugative metabolism of plasmid DNA in the recipients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329943
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