ISSN:
1573-5044
Keywords:
Artemisia annua
;
artemisinin
;
hairy root culture
;
stigmasterol
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Scaled-up hairy root culture of Artemisia annua L. was established in three-liter Erlenmeyer flask. Both artemisinin and stigmasterol that derive from the common precursors of isopentenyl diphosphate and farnesyl pyrophosphate were isolated from hairy roots. The production rate of artemisinin isolated by column chromatography from hairy root cultures was 0.54% (mg.gDW−1). Stigmasterol was identified by mass spectrometry and nuclear magnetic resonance analysis. The production of stigmasterol isolated by column chromatography from hairy root cultures was 108.3% (mg.gDW−1). In hairy root cultures, the production rate of stigmasterol was estimated to be 201 times greater than that of artemisinin. Our results suggest that investigation of secondary metabolites may provide a new insight to study artemisinin production in hairy root cultures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006438919841
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