Publication Date:
1997-08-29
Description:
Newly assembled major histocompatibility complex (MHC) class I molecules, together with the endoplasmic reticulum chaperone calreticulin, interact with the transporter associated with antigen processing (TAP) through a molecule called tapasin. The molecular cloning of tapasin revealed it to be a transmembrane glycoprotein encoded by an MHC-linked gene. It is a member of the immunoglobulin superfamily with a probable cytoplasmic endoplasmic reticulum retention signal. Up to four MHC class I-tapasin complexes were found to bind to each TAP molecule. Expression of tapasin in a negative mutant human cell line (220) restored class I-TAP association and normal class I cell surface expression. Tapasin expression also corrected the defective recognition of virus-infected 220 cells by class I-restricted cytotoxic T cells, establishing a critical functional role for tapasin in MHC class I-restricted antigen processing.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ortmann, B -- Copeman, J -- Lehner, P J -- Sadasivan, B -- Herberg, J A -- Grandea, A G -- Riddell, S R -- Tampe, R -- Spies, T -- Trowsdale, J -- Cresswell, P -- AI30581/AI/NIAID NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1997 Aug 29;277(5330):1306-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, 310 Cedar Street, New Haven, CT 06510, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9271576" target="_blank"〉PubMed〈/a〉
Keywords:
ATP-Binding Cassette Transporters/*metabolism
;
Amino Acid Sequence
;
Antigen Presentation
;
Antiporters/chemistry/genetics/*metabolism
;
Calcium-Binding Proteins/metabolism
;
Calreticulin
;
Cell Line
;
Cell Line, Transformed
;
Chromosome Mapping
;
Chromosomes, Human, Pair 6
;
Cloning, Molecular
;
Dimerization
;
Endoplasmic Reticulum/metabolism
;
Genetic Linkage
;
HLA Antigens/*metabolism
;
Histocompatibility Antigens Class I/*metabolism
;
Humans
;
Immunoglobulin G/chemistry
;
Immunoglobulins/chemistry/genetics/*metabolism
;
Major Histocompatibility Complex/genetics
;
Membrane Transport Proteins
;
Molecular Sequence Data
;
Ribonucleoproteins/metabolism
;
Sequence Homology, Amino Acid
;
T-Lymphocytes, Cytotoxic
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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