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  • 1
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    RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs (2002)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2002-01-26
    Description: Posttranscriptional gene silencing in Caenorhabditis elegans results from exposure to double-stranded RNA (dsRNA), a phenomenon designated as RNA interference (RNAi), or from co-suppression, in which transgenic DNA leads to silencing of both the transgene and the endogenous gene. Here we show that single-stranded RNA oligomers of antisense polarity can also be potent inducers of gene silencing. As is the case for co-suppression, antisense RNAs act independently of the RNAi genes rde-1 and rde-4 but require the mutator/RNAi gene mut-7 and a putative DEAD box RNA helicase, mut-14. Our data favor the hypothesis that gene silencing is accomplished by RNA primer extension using the mRNA as template, leading to dsRNA that is subsequently degraded.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tijsterman, Marcel -- Ketting, Rene F -- Okihara, Kristy L -- Sijen, Titia -- Plasterk, Ronald H A -- New York, N.Y. -- Science. 2002 Jan 25;295(5555):694-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Hubrecht Laboratory, Center for Biomedical Genetics, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11809977" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Motifs ; Animals ; Caenorhabditis elegans/embryology/enzymology/*genetics ; Caenorhabditis elegans Proteins/chemistry/genetics/*metabolism ; Carrier Proteins/genetics/metabolism ; DEAD-box RNA Helicases/chemistry/genetics/*metabolism ; *Gene Silencing ; *Genes, Helminth ; Genes, Reporter ; Green Fluorescent Proteins ; Luminescent Proteins/genetics/metabolism ; Mutation ; Oligoribonucleotides/genetics ; RNA Helicases/chemistry/genetics/*metabolism ; RNA, Antisense/*genetics ; RNA, Double-Stranded/genetics ; RNA, Helminth/genetics/metabolism ; RNA, Messenger/genetics/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Secondary siRNAs result from unprimed RNA synthesis and form a distinct class (2006)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2006-12-13
    Description: In Caenorhabditis elegans, an effective RNA interference (RNAi) response requires the production of secondary short interfering RNAs (siRNAs) by RNA-directed RNA polymerases (RdRPs). We cloned secondary siRNAs from transgenic C. elegans lines expressing a single 22-nucleotide primary siRNA. Several secondary siRNAs start a few nucleotides downstream of the primary siRNA, indicating that non-RISC (RNA-induced silencing complex)-cleaved mRNAs are substrates for secondary siRNA production. In lines expressing primary siRNAs with single-nucleotide mismatches, secondary siRNAs do not carry the mismatch but contain the nucleotide complementary to the mRNA. We infer that RdRPs perform unprimed RNA synthesis. Secondary siRNAs are only of antisense polarity, carry 5' di- or triphosphates, and are only in the minority associated with RDE-1, the RNAi-specific Argonaute protein. Therefore, secondary siRNAs represent a distinct class of small RNAs. Their biogenesis depends on RdRPs, and we propose that each secondary siRNA is an individual RdRP product.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sijen, Titia -- Steiner, Florian A -- Thijssen, Karen L -- Plasterk, Ronald H A -- New York, N.Y. -- Science. 2007 Jan 12;315(5809):244-7. Epub 2006 Dec 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Hubrecht Laboratory (NIOB-KNAW), Uppsalalaan 8, 3584 CT, the Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17158288" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Genetically Modified ; Base Pairing ; Caenorhabditis elegans/*genetics/metabolism ; Caenorhabditis elegans Proteins/metabolism ; Cloning, Molecular ; Phosphates/analysis ; *RNA Interference ; RNA Replicase/metabolism ; RNA, Antisense/*biosynthesis/chemistry/metabolism ; RNA, Complementary/biosynthesis ; RNA, Helminth/*biosynthesis/genetics/metabolism ; RNA, Messenger/genetics/metabolism ; RNA, Small Interfering/*biosynthesis/chemistry/metabolism ; Ribonuclease III/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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    PAPER CURRENT
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