Publication Date:
2023-07-22
Description:
In March/April 2018 during a cruise on R/V Sally Ride, SR1805, 15N-NH4+ incubations in 60mL glass serum bottles were performed to measure ammonium oxidation rates to nitrite and nitrous oxide in different depth at 3 different stations in the oxygen deficient zone (ODZ) of the Eastern Tropical North Pacific off the coast of Mexico. Water samples were collected from 30L Niskin bottles deployed with a conductivity-temperature-depth profiler (CTD, Seabird Electronics). The goal was to get a better understanding on the controls of nitrous oxide (N2O) production. The N2O production rate experiments were performed according to Bourbonnais et al. 2021 (https://doi.org/10.3389/fmars.2021.611937). Furthermore, ammonium (NH4+), nitrite (NO2-) and nitrate (NO3-) as well as N2O concentrations were determined using standard fluorometric (Holmes et al. 1999, https://doi.org/10.1139/f99-128), photometric (Strickland and Parsons 1972, hdl:10013/epic.46454.d001), chemiluminescent (Braman and Hendrix 1989, doi:10.1021/ac00199a007) and mass spectrometric techniques (McIlvin and Casciotti 2010, https://doi.org/10.4319/lom.2010.8.54), respectively. The N2O yield per nitrite produced was calculated. The archaeal ammonia monooxygenase gene subunit A (amoA) copy numbers/mL were determined using qPCR as described previously (Peng et al. 2015, https://doi.org/10.1002/2015GB005278).
Keywords:
15N-tracer; 15N tracer incubations (Bourbonnais et al. 2021); Ammonium; Ammonium, labelled, fraction; Ammonium, oxidation rate; Ammonium, oxidation rate, standard error; ammonium oxidation; amoA gene, copy number; amoA gene, copy number, standard deviation; Bottle number; Calculated; Cast number; Chemiluminescence detection (Braman and Hendrix 1989); Comment; CTD, Sea-Bird; CTD/Rosette; CTD-RO; DATE/TIME; Density, sigma-theta (0); Depth, bottom/max; DEPTH, water; eastern tropical north pacific; Event label; Fluorometry (Holmes et al. 1999); greenhouse gas; Identification; LATITUDE; LONGITUDE; Mass spectrometry (McIlvin and Casciotti 2010); N2O production rates; Nitrate; Nitrite; nitrogen cycle; Nitrous oxide, dissolved; Nitrous oxide, hybrid; Nitrous oxide, hybrid, standard error; Nitrous oxide, standard deviation; Nitrous oxide, yield; Nitrous oxide, yield, standard error; Nitrous oxide production; Nitrous oxide production, standard error; North Pacific Ocean; ocean; Oxygen; Photometry (Strickland & Parsons, 1972); Radiation, photosynthetically active; Real-time quantitative polymerase chain reaction (qPCR); Salinity; Sally Ride; SR1805; SR1805_PS1_CTD16; SR1805_PS1_CTD5; SR1805_PS2_CTD32; SR1805_PS2_CTD45; SR1805_PS3_CTD71; SR1805_PS3_CTD84; Station label; STOX; Switchable trace oxygen sensor; Temperature, water; Treatment
Type:
Dataset
Format:
text/tab-separated-values, 796 data points
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