ISSN:
1476-5535
Keywords:
Dendrogram
;
Insecticidal strain
;
Multilocus pattern
;
Polyacrylamide gel electrophoresis
;
Microbial insecicide
;
Bacillus sphaericus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Analysis of aminopeptidase activity in commercially available enzyme panels (SAP-II and SAP-III) indicated qualitative differences among the eight strains ofBacillus sphaericus tested. These differences involved specific substrates utilized, the importance of sampling time, as well as differences between whole cells and cell-free extracts. Further detailed analysis of four of thel-amino acid-β-naphthylamide substrates using polyacrylamide gel electrophoresis (PAGE) demonstrated a series of allelic differences in the multilocus patterns observed. The resulting phenogram constructed from the enzyme and PAGE information showed a marked separation of the noninsecticidal strains from the insecticidal strains (58% similarity). In addition, there was a separation within the insecticidal strains of strain SSII-1, phage type 2 from strain 2297, phage type 4, and of both of these strains from the remaining highly insecticidal members of phage type 3 (strains 1593, 2013-4, 2362, 2500).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01569431
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