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  • 1
    Electronic Resource
    Electronic Resource
    Identification of CRE1 as a cytokinin receptor from Arabidopsis (2001)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 409 (2001), S. 1060-1063 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cytokinins are a class of plant hormones that are central to the regulation of cell division and differentiation in plants. It has been proposed that they are detected by a two-component system, because overexpression of the histidine kinase gene CKI1 induces typical cytokinin responses and ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35059117
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of gene expression profiles in Arabidopsis salt overly sensitive mutants sos2−1 and sos3 −1 (2005)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 28 (2005), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Under salt-stress conditions, Arabidopsis salt overly sensitive (sos) mutants show hypersensitive root growth. The sos gene products are involved in ion transport and signalling processes. To analyse salt-stress-responsive genes whose transcripts are regulated by the SOS signalling pathway, we used a 22K Agilent oligoarray and quantitative real-time polymerase chain reaction analyses. The amounts of transcripts of the major salt-inducible genes (RD29A/COR78, RD17/COR47, COR15A, KIN1, and RD22) in sos mutants were similar to those in the wild-type plants. These results indicate that the SOS3/SOS2 signalling pathway is independent of the DRE/CRT, ABRE, and MYC/MYB pathways. In sos2 mutants, the expression levels of 27 and 48 genes were up-regulated at least double under 2-h and 5-h salt stress conditions, respectively. Among the genes regulated by SOS2, we detected several encoding myb family transcription factors, AP2 transcription factors, and some ethylene-related, auxin-induced, and defence-related genes. On the other hand, expression levels of very few genes were different between sos3 mutant and wild-type plants. This observation indicates that the downstream SOS2 affects the regulation of stress-responsive genes much more strongly than the upstream SOS3. The gene expression profiles of sos2 and sos3 mutants were not identical. This indicates that SOS2 and SOS3 have individual roles in salt stress responses in addition to common roles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.2005.01363.x
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  • 3
    Electronic Resource
    Electronic Resource
    Bombardment-mediated transformation of plant cells (1994)
    Springer
    Journal of plant research 107 (1994), S. 117-123 
    Details
    ISSN: 1618-0860
    Keywords: Integration ; Organelle ; Particle gun ; Pollen ; Transformation ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Significance of bombardment-mediated transformation in plant research is discussed. Apart from general subjects related to this transformation study, a particular emphasis has been placed on the pollen and organelle transformation and on integration mechanism. Over 70 literatures related to this field are cited here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346006
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  • 4
    Electronic Resource
    Electronic Resource
    Transient expression of β-glucuronidase in plastids of various plant cells and tissues delivered by a pneumatic particle gun (1995)
    Springer
    Journal of plant research 108 (1995), S. 235-240 
    Details
    ISSN: 1618-0860
    Keywords: α-amanitin ; β-glucuronidase ; Particle gun ; Plastid transformation ; Transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast expression plasmids pTRBCL-GUS (tobaccorbcL promoter-gusA-tobaccorbcL terminator) and pHHU3004 (spinach ‘x gene’ promoter-gusA-spinachrbcL terminator) and a control nuclear expression plasmid pBI221 (CaMV 35S promoter-gusA-NOS terminator) were introduced separately into cultured cells and tissues of tobacco andArabidopsis thaliana, as well as into cultured cells of the lower land plants liverwort and hornwort by a pneumatic particle gun. The pTRBCL-GUS and pHHU3004 plasmids produced many blue spots in the BY-2 cells and the roots ofArabidopsis thaliana, but not in any of the green cells or tissues. The results suggest that the pTRBCL-GUS and pHHU3004 plasmids are expressed more in proplastids and amyloplasts than in chloroplasts. GUS activities of the BY-2 cells bombarded with pTRBCL-GUS and pHHU3004 were insensitive to α-amanitin treatment (10 and 50 μg/ml), while that of the cells with pBI221 greatly decreased by the same treatment. Hence, it is likely that the pTRBCL-GUS and pHHU3004 plasmids were substantially expressed in the proplastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344348
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  • 5
    Electronic Resource
    Electronic Resource
    Transgenic Arabidopsis thaliana plants obtained by particle-bombardment-mediated transformation (1991)
    Springer
    Applied microbiology and biotechnology 36 (1991), S. 228-230 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Stable transformation of Arabidopsis thaliana with plasmid DNA (pCaMVNEO) harbouring the neomycin phosphotransferase II (npt-II) gene was achieved using a previously described pneumatic particle gun device driven by compressed air. Transgenic A. thaliana plants were regenerated from root sections bombarded with DNA-coated gold particles accelerated by the device. Enzyme assays and Southern blot hybridization confirmed the expression of the foreign gene and its stable integration into the Arabidopsis genome. The analysis for kanamycin resistance in R 1 plants from a self-pollinated transformant indicated transmission of the npt-II gene to R 1 progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00164425
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  • 6
    Electronic Resource
    Electronic Resource
    Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment (1995)
    Springer
    Plant molecular biology 28 (1995), S. 337-341 
    Details
    ISSN: 1573-5028
    Keywords: Lilium longiflorum ; Freesia refracta ; Tulipa gesneriana ; GUS mRNA ; particle bombardment ; pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gold particles coated with β-glucuronidase (GUS) mRNA with a 5′ cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020252
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  • 7
    Electronic Resource
    Electronic Resource
    Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression (2000)
    Springer
    Plant molecular biology 42 (2000), S. 657-665 
    Details
    ISSN: 1573-5028
    Keywords: DREB ; DRE ; Arabidopsis ; dehydration ; high salt stress ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In plants, a cis-acting element, DRE/CRT, is involved in ABA-independent gene expression in response to dehydration and low-temperature stress. To understand signal transduction pathways from perception of the dehydration stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB2A and DREB2B in Arabidopsis thaliana. Northern analysis showed that both genes are induced by dehydration and high-salt stress. Organ-specific northern analysis with gene-specific probes showed that these genes are strongly induced in roots by high-salt stress and in stems and roots by dehydration stress. The DREB2A gene is located on chromosome 5, and DREB2B on chromosome 3. We screened an Arabidopsis genomic DNA library with cDNA fragments of DREB2A and DREB2B as probes, and isolated DNA fragments that contained 5′-flanking regions of these genes. Sequence analysis showed that both genes are interrupted by a single intron at identical positions in their leader sequence. Several conserved sequences were found in the promoter regions of both genes. The β-glucuronidase (GUS) reporter gene driven by the DREB2 promoters was induced by dehydration and high-salt stress in transgenic Arabidopsis plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006321900483
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  • 8
    Electronic Resource
    Electronic Resource
    Transient expression of β-glucuronidase in Arabidopsis thaliana leaves and roots and Brassica napus stems using a pneumatic particle gun (1991)
    Springer
    Plant molecular biology 17 (1991), S. 259-263 
    Details
    ISSN: 1573-5028
    Keywords: Gene transfer ; Arabidopsis thaliana ; Brassica napus ; transient expression ; pneumatic particle gun
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Successful transient expression of β-glucuronidase (GUS) in Arabidopsis thaliana leaves and roots and Brassica napus stems was obtained after gene delivery with a pneumatic particle gun driven by compressed air. Effects of the pneumatic pressure used to accelerate the particles (accelerating pressure; 85 to 200 kg/cm2) and of preculture periods of plant tissues (0 to 6 days) on the efficiency of gene delivery were studied. In A. thaliana leaves, best results were obtained at 115 kg/cm2 of accelerating pressure and 3 days of preculture. In A. thaliana roots, the optimum was at 200 kg/cm2 of accelerating pressure and 3 days of preculture. These results indicate that both preculture period and accelerating pressure are vital factors that determine the efficiency of gene delivery by particle gun.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039501
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  • 9
    Electronic Resource
    Electronic Resource
    Stable transformation ofArabidopsis with thebar gene using particle bombardment (1994)
    Springer
    Transgenic research 3 (1994), S. 279-286 
    Details
    ISSN: 1573-9368
    Keywords: Arabidopsis thaliana ; transgenic plants ; particle bombardment ; bar gene ; phosphinothricin acetyltransferase ; late-flowering mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A plasmid pARK 22 harbouring thebar gene encoding phosphinothricin acetyltransferase (PAT) under the control of the cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator was constructed and introduced into root sections ofArabidopsis thaliana using the pneumatic particle gun. The root sections that had been bombarded with this plasmid gave four to eight times higher yield of drug-resistant calluses than those sections bombarded with pCaMVNEO or pCH, which respectively contain the neomycin phosphotransferase and hygromycin phosphotransferase genes. Among a number of primary transformant (T0) plants obtained from independent bialaphos-resistant calluses, three were studied by Southern blot hybridization and PAT enzyme activity analyses, confirming the stable integration of the foreign gene into theArabidopsis genome and its expression in plants. The progeny analysis showed transmission of the foreign gene and its expression in up to the T2 generation. Some of the T1 progeny showed morphological abnormalities. Thus, thebar gene can be used effectively to allow selection of transgenicA. thalianna plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01973587
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  • 10
    Electronic Resource
    Electronic Resource
    Transgenic haploid plants ofNicotiana rustica produced by bombardment-mediated transformation of pollen (1995)
    Springer
    Transgenic research 4 (1995), S. 341-348 
    Details
    ISSN: 1573-9368
    Keywords: transgenic haploid plants ; pollen culture ; particle bombardment ; β-glucuronidase ; neomycin phosphotransferase II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immature pollen fromNicotiana rustica was bombarded with gold particles coated with plasmid DNA encoding neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) genes which, respectively, are under the control of the cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator in the plasmid. Kanamycin-resistant pollen embryoids were selected from the bombarded pollen cells and two independent lines of transgenic plants were regenerated. Enzyme assays showed that one has both NPTII and GUS activities and the other only weak NPTII activity. Southern blot analyses indicated that the former has a DNA fragment corresponding to the intact expression cassettes for both genes in its genome; whereas the latter lacks intact expression cassettes for both genes and has only the intactnptII coding sequence in its genome. The transgenic plants of both lines have 24 chromosomes, confirming haploidy, and they are infertile. These results indicate that transgenic haploid plants can be produced directly by the bombardment-mediated transformation of immature pollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972531
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