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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 62 (1997), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A test chamber was designed and constructed and prechill chicken carcasses inoculated with Salmonella typhimurium were treated in it. They were sprayed with tap water, 0.85% sodium chloride (NaCl), 5% or 10% trisodium phosphate (TSP), 5% or 10% sodium bisulfate (SBS), 0.1% cetylpyridinium chloride (CPC), or 1% lactic acids (LAC) at 207, 345 or 827 kPa pressure for either 30 or 90 sec exposure time. Samples were taken from carcass wash water to determine the most probable number of Salmonella. Compared to tap water spraying, 0.85% NaCl spraying did not significantly reduce Salmonella. The greatest reductions of S. typhimurium, by 10% TSP, 10% SBS, 0.1% CPC or 1% LAC spraying, were 3.7, 2.4, 1.6 or 1.6 log in 90 sec treatments, respectively.
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 63 (1998), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Chemical spray parameters, including temperature, pressure and exposure time, were evaluated for their effects on reducing Salmonella typhimurium on chicken skins. Pre-chilled chicken carcass skins were inoculated with S. typhimurium and sprayed with 0.1% cetylpyridinium chloride (CPC), 10% trisodium phosphate (TSP), or 2% lactic acid (LA). In the CPC spray, the 40°C treatments resulted in the greatest bacterial reduction. The most effective spray temperatures for LA and TSP treatments were 40 to 55°C. All chemical spray treatments at 207–1034 kPa reduced S. typhimurium. The reduction of S. typhimurium was greatest in all treatments when sprayed with 90 sec spray and 90 sec setting time.
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  • 3
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Chicken skins inoculated with Salmonella typhimurium at 1 × 108 CFU/mL were subjected to chemical or electrical treatments (4 mA/ cm2 current, 1 kHz frequency and 50% duty cycle) for 10 min in 1% sodium chloride (NaCl), sodium carbonate (Na2CO3), or trisodium phosphate (Na3PO4 12H2O or TSP). Salmonellae on chicken skins and in treatment solutions and rinsing water were enumerated with microbiological platings. Chicken skin was also examined using scanning electron microscopy.S. typhimurium attached to skins were reduced by 90% after electrical treatments in 1% NaCl, Na2CO3, or TSP, while the reduction ranged from 34% to 76% in groups treated by the salts alone.
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 4 (1996), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A membrane lifting technique was developed for direct rapid detection of Escherichia coli 0157:H7 in inoculated ground meats. Duplicate groups of 2 meatballs were inoculated with volumes of 0.1-ml of a serial dilution (1:10) of E. coli 0157:H7 or a mixed culture containing one strain of E. coli 0157:H7 and a non-0157:H7E. coli serotype (E. coli ATCC 25922). Each meatball was sampled by sandwiching with 2 pieces of nitrocellulose membranes and pressing against each other to the center of the meatball. The membranes were in contact with the meats for 10 min to lift the bacteria. The membranes were removed and incubated on MacConkey-sorbitol agar plates with the meat contact side up. After 18 h incubation at 37C, an immunostain was performed directly on the membranes for detection of the presence or absence of E. coli 0157:H7. This method was found to be sensitive enough to detect as few as 1 to 2 cells of E. coli 0157:H7 inoculated on surfaces of 18-g meatballs. This method might be used as a rapid presumptive test for E. coli 0157:H7.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1994), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mycoplasma gallisepticum infection in chickens was detected with a fluorescence concentration immunoassay (FCIA) using intact mycoplasmal cells as the reacting particles. The antigen and test chicken antisera were directly pipetted into the wells of a 96-well fluoricon assay plate and incubated 30 min at ambient temperature in the chamber of a fluorescence concentration analyzer. The antigen-antibody bound complexes and free components automatically were separated by vacuum filtrations and washings through the membrane filter equipped at the base of the wells. Fluorescein isothiocyanate (FITC) was used as the tracer via FITC-goat-anti-chicken IgG which was incubated with the above formed antigen-antibody complexes in the wells for another 30 min. After the vacuum filtrations and washings, the fluorescent signal was read at a wavelength of 485/535 nm and was reported in a numerical arbitrary fluorescence unit (AFU). The positive cutoff AFU value was calculated by adding 2 standard deviations to the average AFU of the negative control. Samples with AFU above the cut-off value were scored positive indicating the infection. It was found the FCIA method was comparable in sensitivity to the Agritech ELISA method and more sensitive than the serum plate agglutination (SPA) test or hemagglutination-inhibition (HI) test. Forty-seven/51 sera from M. gallisepticum inoculated chickens were scored positive using FCIA or ELISA methods, while 36/51 and 40/51 were indicated positive by HI and SPA tests respectively. The specificity of the FCIA assay using sera from M. gallisepticum inoculated chickens was shown to be comparable to the HI test. Cross-reactivity occurred at 35%, 8% and 11% in SPA, HI and FCIA tests respectively. The FCIA assay can be completed in less than 2 h with a total of 24 samples including one negative control and one positive control sera assayed in one plate.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 10 (2002), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A DNA binding fluorescence method based on polymerase chain reaction (PCR) products was evaluated for rapid detection of Salmonella Typhimurium in poultry products. Wash water samples of chicken carcasses and ground turkey were inoculated with S. Typhimurium to obtain final concentrations of 10° - 105 CFU/mL. One mL of each sample was used to get the DNA template and 5 μL of the sample template was added into 25 μL of SYBR Green PCR Master Mix and two specific Salmonella ompC gene primers. The negative control was the same except 5 μL of each wash solution was added instead of 5 μL sample template. The reaction was carried out in a thermocycler. Finally, the fluorescence signal of each PCR product was measured using a fluorometer. The PCR products were also confirmed by ethidium bromide agarose gel, and the DNA concentrations of the PCR products were measured by a filter fluorescence photometer. The results showed that when bacterial cells increased from 0 to 2 CFU/mL, the fluorescence signal increased significantly. The PCR-based fluorescence method could detect the target bacteria in minutes after PCR amplification compared to hours by gel electrophoresis and also could be done at an earlier time during PCR amplification. The detection limit of this method for S. Typhimurium in the poultry samples was 2 CFU/mL without any enrichment.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food safety 11 (1990), S. 0 
    ISSN: 1745-4565
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A rapid, sensitive nitrocellulose membrane lifting technique was developed to identify Salmonella typhimurium attached to chicken skin. Basically, this method used a nitrocellulose membrane to remove salmonellae attached to chicken skin. The membrane with attached salmonellae was then incubated on xylose lysine deoxycholate (XLD) agar for 18 h at 37°C. Appearance of black colonies on the white membrane was considered a positive presumptive test for salmonellae. Immunostaining of colonies on the nitrocellulose membrane was then used to confirm presence or absence of salmonellae. This technique can be used to identify salmonellae contamination of chicken skin in less than 24 h and was shown to be more sensitive than presently used swabbing or washing techniques.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 1 (1992), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: DNA probes specific for Campylobacter jejuni have been developed. A genomic library of C. jejuni was constructed and screened with 32P-labeled genomic DNA from C. jejuni and C. coli by colony hybridization. Several clones that reacted only with C. jejuni but not with C. coli were selected. Two DNA inserts (1.5 kb and 0.8 kb) were isolated from the positive clones. A modified Southern hybridization technique indicated that these two 32P-labeled DNA inserts were specific for C. jejuni. the 1.5 kb DNA insert was partly sequenced, and a 19 base oligomer probe was selected from the sequences. Southern hybridization indicated that the oligomer probe hybridized with DNA from all seven strains of C. jejuni tested bus did not hybridize with DNA from four strains of C. coli, one strain of C. fetus and C. laridis, or 11 other bacterial species tested.
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 1 (1992), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A rapid polymerase chain reaction (PCR) method was developed for detection of low numbers of Campylobacter jejuni. In this method, PCR was run directly from lysed C. jejuni cells and used a short denaturing and annealing time, a rapid transition, and an increased number of cycles to obtain good sensitivity. Only 3 h were required for PCR and 1 h for electrophoresis. Additional time for DNA isolation and DNA hybridization was not needed. This system was positive for C. jejuni but negative for C. coli and C. fetus. the method detected as few as two cells in original liquid ofC. jejuni in pure cultures. an internal probe hybridization test confirmed that the PCR products were from C. jejuni.
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 1 (1992), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two monoclonal antibodies (AMb) that react only with Mycoplasma gallisepticum (MG) and do not cross-react with Mycoplasma synoviae (MS) were established. MAb 76 was IgG1 and reacted with a 66 kDa MG protein. MAb 69 was IgG2b and reacted with a 100 kDa MG protein. Using these MAbs as positive controls in an immunoblotting test, 9 of 12 sera from MG inoculated chickens gave a strong band at 66 kDa, and no bands were found at 66 kDa using either 11 sera from MS inoculated chickens or 10 sera from uninoculated, negative control chickens. These results indicated that the 66 kDa protein is a major species-specific protein for MG and that the MAb 76 is a major species-specific monoclonal antibody for MG. the MAb 76 identified 7 of 7 M. gallisepticum strains by a cell-dot-blot method. A coagglutination test using MAb 76 bound to protein A positive Staphylococcus aureus cells was also shown to be a rapid method to identify the seven strains of M. gallisepticum.
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