Publication Date:
1988-09-02
Description:
When two different mammalian cell types are fused to generate a stable hybrid cell line, genes that are active in only one of the parents are frequently shut off, a phenomenon called extinction. In this study two distinct, complementary mechanisms for such extinction of growth hormone gene expression were identified. In hybrids formed by fusing fibroblasts to pituitary cells, pituitary-specific proteins that bind to the growth hormone promoter were absent. In addition, a negative regulatory element located near the rat growth hormone promoter was specifically activated.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tripputi, P -- Guerin, S L -- Moore, D D -- New York, N.Y. -- Science. 1988 Sep 2;241(4870):1205-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Massachusetts General Hospital, Boston.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2842865" target="_blank"〉PubMed〈/a〉
Keywords:
Acetyltransferases/genetics
;
Animals
;
Avian Sarcoma Viruses/genetics
;
Chloramphenicol O-Acetyltransferase
;
Enhancer Elements, Genetic
;
Fibroblasts/metabolism
;
*Gene Expression Regulation
;
Growth Hormone/*genetics
;
Herpesviridae/genetics
;
Hybrid Cells/*metabolism
;
Hypoxanthine Phosphoribosyltransferase/genetics
;
L Cells (Cell Line)
;
Mice
;
Pituitary Gland/metabolism
;
Plasmids
;
Promoter Regions, Genetic
;
Rats
;
Thymidine Kinase/genetics
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink