Abstract
When two different mammalian cell types are fused to generate a stable hybrid cell line, genes that are active in only one of the parents are frequently shut off, a phenomenon called extinction. In this study two distinct, complementary mechanisms for such extinction of growth hormone gene expression were identified. In hybrids formed by fusing fibroblasts to pituitary cells, pituitary-specific proteins that bind to the growth hormone promoter were absent. In addition, a negative regulatory element located near the rat growth hormone promoter was specifically activated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetyltransferases / genetics
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Animals
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Avian Sarcoma Viruses / genetics
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Chloramphenicol O-Acetyltransferase
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Enhancer Elements, Genetic
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Fibroblasts / metabolism
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Gene Expression Regulation*
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Growth Hormone / genetics*
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Herpesviridae / genetics
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Hybrid Cells / metabolism*
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Hypoxanthine Phosphoribosyltransferase / genetics
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L Cells
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Mice
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Pituitary Gland / metabolism
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Plasmids
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Promoter Regions, Genetic
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Rats
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Thymidine Kinase / genetics
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Transfection
Substances
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Growth Hormone
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Acetyltransferases
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Chloramphenicol O-Acetyltransferase
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Hypoxanthine Phosphoribosyltransferase
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Thymidine Kinase