Publication Date:
2003-07-19
Description:
Direct quantification of biomolecular interaction by single-molecule force spectroscopy has evolved into a powerful tool for materials and life sciences. We introduce an approach in which the unbinding forces required to break intermolecular bonds are measured in a differential format by comparison with a known reference bond (here, a short DNA duplex). In addition to a marked increase in sensitivity and force resolution, which enabled us to resolve single-base pair mismatches, this concept allows for highly specific parallel assays. This option was exploited to overcome cross-reactions of antibodies in a protein biochip application.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Albrecht, Christian -- Blank, Kerstin -- Lalic-Multhaler, Mio -- Hirler, Siegfried -- Mai, Thao -- Gilbert, Ilka -- Schiffmann, Susanne -- Bayer, Tom -- Clausen-Schaumann, Hauke -- Gaub, Hermann E -- New York, N.Y. -- Science. 2003 Jul 18;301(5631):367-70.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Nanotype GmbH, Lochhamer Schlag 12, 82166 Grafelfing, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12869761" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies
;
*Base Pair Mismatch
;
*Biosensing Techniques
;
Carbocyanines
;
Cross Reactions
;
*DNA/chemistry/genetics/metabolism
;
Dimethylpolysiloxanes
;
Fluorescence
;
Fluorescent Dyes
;
Glass
;
Humans
;
Immunoassay
;
Interleukin-5/analysis/immunology
;
Mice
;
Microscopy, Atomic Force
;
Nucleic Acid Conformation
;
Nucleic Acid Hybridization
;
Oligodeoxyribonucleotides/chemistry/metabolism
;
*Oligonucleotide Array Sequence Analysis
;
*Protein Array Analysis
;
Protein Binding
;
Silicones
;
Temperature
;
Thermodynamics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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