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The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles (1995)

Sanz-Alferez, S. ; Richter, T. E. ; Hulbert, S. H. ; [et al.]

Springer

Theoretical and applied genetics 91 (1995), S. 25-32

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ISSN: 1432-2242

Keywords: Restriction fragment length polymorphism ; Near-isogenic lines ; Puccinia sorghi ; Introgression ; Gene duplication

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The Rp3 locus of maize conditions race-specific resistance to a fungal rust pathogen, Puccinia sorghi. Both morphological and DNA markers were employed to characterize alleles of Rp3 and to accurately position Rp3 on the maize genetic map. DNA marker polymorphisms distinctive to each Rp3 allele were identified, allowing the identification of specific Rp3 alleles in cases where rust races that differentiate particular alleles are not available. In a population of 427 progeny, Rp3 and Rg1 were found to be completely linked, while Lg3 was approximately 3 cM proximal on the long arm of chromosome 3. In this same population, 12 RFLP markers were mapped relative to Rp3; the closest markers were UMC102 (about 1cM distal to Rp1) and NPI114 (1–2 cM proximal). These and additional DNA probes were used to characterize the nature and extent of flanking DNA that was carried along when six different Rp3 alleles were backcrossed into a single background. Depending upon the allele investigated, a minimum of 2–10cM of polymorphic DNA flanking the Rp3 locus was retained through the introgression process. In addition, many of the probes that map near Rp3 were found to detect an additional fragment in the Rp3 region, indicating that portions of this chromosomal segment have been tendemly duplicated. The materials and results generated will permit marker-assisted entry of Rp3 into different maize backgrounds and lay the foundation for the eventual map-based cloning of Rp3.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00220854

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Complex duplications in maize lines (1993)

Hong, K. S. ; Richter, T. E. ; Bennetzen, J. L. ; [et al.]

Springer

Molecular genetics and genomics 239 (1993), S. 115-121

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ISSN: 1617-4623

Keywords: RFLP ; Disease resistance ; Duplicated sequence ; Recombination ; Linked repeats ; Hypervariable loci

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Rp1 is a disease resistance complex and is the terminal morphological marker on the short arm of maize chromosome 10. Several restriction fragment length polymorphisms (RFLPs), which map within 5 map units of Rp1, were examined to determine if they are also complex in structure. Two RFLP loci, which mapped distally to Rp1, BNL3.04 and PIO200075, existed in a single copy in all maize lines examined. These two loci cosegregated perfectly in 130 test cross progeny. Two RFLP loci that map proximally to Rp1 had unusual structures, which have not yet been reported for maize RFLPs; the loci were complex, with variable numbers of copies in different maize lines. One of the loci, NPI285, occasionally recombined in meiosis to yield changes in the number of copies of sequences homologous to the probe. The other proximal locus, detected by the probes NPI422, KSU3, and KSU4, was relatively stable in meiosis and no changes in the number of restriction fragments were observed. The similarity in map position between Rp1 and the complex RFLP loci indicate there may be genomic areas where variable numbers of repeated sequences are common. The structure of these complex loci may provide insight into the structure and evolution of Rp1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00281609

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