Abstract
Rp1 is a disease resistance complex and is the terminal morphological marker on the short arm of maize chromosome 10. Several restriction fragment length polymorphisms (RFLPs), which map within 5 map units of Rp1, were examined to determine if they are also complex in structure. Two RFLP loci, which mapped distally to Rp1, BNL3.04 and PIO200075, existed in a single copy in all maize lines examined. These two loci cosegregated perfectly in 130 test cross progeny. Two RFLP loci that map proximally to Rp1 had unusual structures, which have not yet been reported for maize RFLPs; the loci were complex, with variable numbers of copies in different maize lines. One of the loci, NPI285, occasionally recombined in meiosis to yield changes in the number of copies of sequences homologous to the probe. The other proximal locus, detected by the probes NPI422, KSU3, and KSU4, was relatively stable in meiosis and no changes in the number of restriction fragments were observed. The similarity in map position between Rp1 and the complex RFLP loci indicate there may be genomic areas where variable numbers of repeated sequences are common. The structure of these complex loci may provide insight into the structure and evolution of Rp1.
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Beavis WD, Grant D (1991) A linkage map based on information from four F2 populations of maize (Zea mays L.). Theor Appl Genet 82:636–644
Bennetzen JL, Qin MM, Ingels S, Ellingboe AH (1988) Allele-specific and Mutator-associated instability at the Rp1 diseaseresistance locus of maize. Nature 332:369–370
Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103
Burr B, Burr FA, Thompson KH, Albertson MC, Stuber CW (1988) Gene mapping with recombinant inbreds of maize. Genetics 118:519–526
Chang C, Bowman JL, DeJohn AW, Landry ES, Meyerowitz EM (1988) Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci USA 85:6856–6860
Das OP, Levi-Minzi S, Koury M, Benner M, Messing JA (1990) Somatic gene rearrangement contributing to genetic diversity in maize. Proc Natl Acad Sci 87:7809–7813
Das OP, Poliak E, Ward K, Messing JA (1991) New allele of the duplicated 27 kD zein locus of maize generated by homologous recombination. Nucl Acids Res 19:3325–3330
Dooner HK, Kermicle JL (1971) Structure of the Rr tandem duplication in maize. Genetics 67:427–436
Dooner HK, Kermicle JL (1976) Displaced and tandem duplications in the long arm of chromosome 10 in maize. Genetics 82:309–322
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
Greenblatt IM (1984) A chromosome replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, Modulator, in maize. Genetics 108:471–485
Helentjaris T, Weber D, Wright S (1988) Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics 118:353–363
Hulbert SH, Bennetzen JL (1991) Recombination at the Rp1 locus of maize. Mol Gen Genet 226:377–382
Hulbert SH, Richter TE, Axtell JD, Bennetzen JL (1990) Genetic mapping and characterization of sorghum and related crops using maize DNA probes. Proc Natl Acad Sci USA 87:4251–4255
Ingels SC, Bennetzen JL, Hulbert SH, Qin MM, Ellingboe AH (1992) Mutator transposable elements that occur in clusters in the maize genome. J Heredity 83:114–118
Johns MA, Fuerstenberg SI, Hennelly CA (1990) Non-random chromosomal distribution of Ac-like sequences in inbred maize. Genet Res Camb 55:71–80
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337
Laughnan JR (1949) The action of allelic forms of the gene A in maize II. The relation of crossing-over to mutation of A b. Proc Natl Acad Sci USA 35:167–178
Liboz T, Bardet C, LeVan Thai A, Axelos M, Lescure B (1989) The four members of the gene family encoding the Arabidopsis thaliana translation elongation factor EF-1a are actively transcribed. Plant Mol Biol 14:107–110
Liu C-N, Rubenstein I (1992) Genomic organization of an a-zein gene cluster in maize. Mol Gen Genet 231:304–312
McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Pang P, Pruitt RE, Meyerowitz EM (1988) Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Mol Biol 11:805–820
Pryor A (1987) The origin and structure of fungal disease resistance in plants. Trends Genet 3:157–161
Robbins TP, Walker EL, Kermicle JL, Alleman M, Dellaporta SL (1991) Meiotic instability of the R-r complex arising from displaced intragenic exchange and intrachromosomal rearrangement. Genetics 129:271–283
Ryder TB, Hedrich SA, Bell JN, Liang X, Clouse SD, Lamb CJ (1987) Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet 210:219–233
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Saxena KMS, Hooker AL (1968) On the structure of a gene for disease resistance in maize. Proc Natl Acad Sci USA 68:1300–1305
Sudupak MA, Bennetzen JL, Hulbert SH (1993) Unequal exchange and meiotic instability of Rp1 region disease resistance genes in maize. Genetics (in press)
Sugita M, Manzara T, Pichersky E, Cashmere A, Gruissem W (1987) Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato. Mol Gen Genet 209:247–256
Tanksley SD, Bernatzky R, Lapitan NL, Prince JP (1988) Conservation of gene repertoire but not gene order in pepper and tomato. Proc Natl Acad Sci 85:6419–6423
Veit B, Vollbrecht E, Mathern J, Hake S (1990) A tandem duplication causes the Kn1-O allele of Knotted, a dominant morphological mutant of maize. Genetics 125:623–631
Weber D, Helentjaris T (1989) Mapping RFLP loci in maize using B-A translocations. Genetics 121:583–590
Yamaguchi-Shinozaki K, Mundy J, Chua N (1989) Four tightly linked rab genes are differentially expressed in rice. Plant Mol Biol 14:29–39
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Communicated by E. Meyerowitz
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Hong, K.S., Richter, T.E., Bennetzen, J.L. et al. Complex duplications in maize lines. Molec. Gen. Genet. 239, 115–121 (1993). https://doi.org/10.1007/BF00281609
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DOI: https://doi.org/10.1007/BF00281609