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  • 1
    Publication Date: 2021-05-19
    Description: This project was designed and producted to determine the optimal level of protein and energy for maximizing growth of fingerling Barbus grypus......
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fish ; Growth ; Fingerlings ; Protein ; Diet
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 84pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Effects of using various ratios of C:N in biofloc system on growth performance, feeding indices and culture water quality of common carp with mean weight of 14.17±0.36 g were investigated during 90 days in experimental treatments including control (no extra carbon source) and four treatments with different C:N ratios of 11, 15, 19 and 23 each with 3 replicates. The increase in C:N ratio had a significant effect on growth and feeding indices of cultured common carp in treatments (p〈0.05). The highest final weight and weight gain, the lowest FCR and the highest SGR were observed in C:N ratio of 19. No significant difference was observed in survival rate among different treatments indicating that the culture condition was suitable for common carp in this biofloc system. Hepatosomatic and visceral indices did not show significant differences between treatments with different C:N ratios (p〉 0.05). Water quality indices including DO, pH, TDS, EC, TSS, FV, TAN, nirtite and nitrate showed significant differences in biofloc system with different C:N ratios (p〈0.05). The mean values of FV and TSS in biofloc system increased with increasing C:N ratio. The variation pattern of the mean TAN and nitrite were the same as each other and reverse to the trend of TSS and FV showed a decreasing trend with increasing C:N ratio. These trends indicate the significant removal of nitrogenous compounds by bioflocs. In conclusion, using C:N ratio of 19 with molasses as carbon source in biofloc system could be recommended to improve water quality, growth and feeding performance of cultured common carp.
    Description: Published
    Keywords: Cyprinus carpio ; Fish ; Biofoc system ; Water quality ; Feeding
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.13-24
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/24968 | 18721 | 2018-11-19 09:48:42 | 24968 | Khorramshahr University of Marine Science and Technology
    Publication Date: 2021-07-16
    Description: Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of the Persian Gulf Oyster (Crassostrea sp.), examined in 30 samples collected from Bandar-e Emam Khomeini. Polymerase chain reaction was performed by a pair of primer for all samples and PCR products were sequenced using dideoxy chain termination method . Then the obtained data were analyzed usig MEGA Ver 5.0, BioEdit Ver 7.0 and DnaSP Ver 5.0 programs and software. The results showed 2 mutation and Nucleotide diversity (Pi) 0.00061 observed in studied genes. Among the 30 samples, 3 haplotypes were detected and average haplotype diversity (Hd) 0.195 were calculated. The haplotypes obtained in this study were submitted in the Gene Bank for the first time. Phylogeny trees derived from 16S rRNA gene sequencing showed no significant separation of the samples in the Imam Khomeini port. The results of this study also showed that there is low sequence differentiation in the study population in Imam Khomeini port.
    Keywords: Biology ; Iran ; Emam Khomayni Port ; 16S rRNA ; Crassostrea ; Primer ; Polymerase chain reaction ; Sequencing ; Genetic ; Diversity
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 14-22
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/24983 | 18721 | 2018-11-17 18:22:04 | 24983 | Khorramshahr University of Marine Science and Technology
    Publication Date: 2021-07-16
    Description: According to the role of the growth hormone gene in the aquaculture, in this research cloning growth hormone coioides (Epinephlus coioides) was evaluated in vector pTZ57R/T. After PCR product purification by kit QIA quick Gel Extraction, growth hormone gene was integrated into pTZ57R/T vector. Constructed vector was transferred in E. coli strain DH5α competent cells. The white colonies was recombinant bacteria, from that that plasmid extraction was performed. Cloning obtained conformed using with direct PCR and sequencing methods. Growth hormone cDNA from E. coioide has an open reading frame of 615 nucleotides and 204 amino acids respectively. The calculated molecular weight and predicted of isoelectric point of the growth hormone protein were 014/23 kDa and 9.6 respectively. The results of this study showed that growth hormone gene in pTZ57R/T vector successfully had been cloned and can be used to growth hormone gene expression and protein production. The operation of gene sequencing with growth hormone gene from E. coioides in the Gen Bank was showed similarity between them.
    Keywords: Biology ; Iran ; Growth ; Hormone ; Cloning ; Epinephelus coioides ; pTZ57R/T vect ; E. coli ; Identification ; Aquaculture ; Gene ; GenBank
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 108-117
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/24867 | 18721 | 2018-11-22 07:06:00 | 24867 | Khorramshahr University of Marine Science and Technology
    Publication Date: 2021-07-15
    Description: Study was conducted to investigate the effects of various energy levels on growth performance, food conversion ratio (FCR) and whole body composition in Gattan, Barbus xanthopterus fingerlings (12.12±0.28 g) for 8 week. Feeding trial was conducted using 3 semipurfied diets containing 3 levels of digestible energy (DE 2.5, 3 and 3.5 kcal/g) and same protein level 35%, in a flow-through system (1L/min) at 26.2 ± 0.460C. Triplicate groups of 15 fish (12.12±0.22 g) each were stocked in 300-L circular polyethylene tanks and fish were fed to satiety thrice daily throughout the feeding trial. The best of total weight gain (146.67 g), FCR (2.21), protein efficiency ratio (1.31), apparent net protein utilization (ANPU %) (34.69) and condition factor (CF) (1.26) were observed in diet containing 3 kcal/g DE. The viscerosomatic index (VSI) and hepatosomatic index (HSI) did not show any significant difference between treatments (P〉0.05). The final whole body moisture content decreased significantly with increasing energy levels in diets (P〈0.05). Although whole body lipid and protein contents did not show significant variation among treatments (P〉0.05), but the highest lipid and protein deposition were observed in diets containing 3.5 and 3kcal/g DE, respectively. Comparison between varying levels of dietary energy on the growth, feed utilization and whole body composition of Gattan indicated that 3 kcal/g DE could be the preferential dietary energy levels in fingerlings of this species.
    Keywords: Aquaculture ; Biology ; Dietary ; Energy ; Level ; Growth ; Whole body composition ; Gattan (B. xanyhopterus) ; Barbus xanthopterus ; Fingerlings ; Feeding ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 53-64
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