Publication Date:
2011-01-15
Description:
Infection of chickens with avian influenza virus poses a global threat to both poultry production and human health that is not adequately controlled by vaccination or by biosecurity measures. A novel alternative strategy is to develop chickens that are genetically resistant to infection. We generated transgenic chickens expressing a short-hairpin RNA designed to function as a decoy that inhibits and blocks influenza virus polymerase and hence interferes with virus propagation. Susceptibility to primary challenge with highly pathogenic avian influenza virus and onward transmission dynamics were determined. Although the transgenic birds succumbed to the initial experimental challenge, onward transmission to both transgenic and nontransgenic birds was prevented.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lyall, Jon -- Irvine, Richard M -- Sherman, Adrian -- McKinley, Trevelyan J -- Nunez, Alejandro -- Purdie, Auriol -- Outtrim, Linzy -- Brown, Ian H -- Rolleston-Smith, Genevieve -- Sang, Helen -- Tiley, Laurence -- BB/G00479X/1/Biotechnology and Biological Sciences Research Council/United Kingdom -- BBS/B/00239/Biotechnology and Biological Sciences Research Council/United Kingdom -- BBS/B/00301/Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2011 Jan 14;331(6014):223-6. doi: 10.1126/science.1198020.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21233391" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Animals, Genetically Modified
;
Cell Line
;
Chickens/*genetics/virology
;
Cloaca/virology
;
Influenza A Virus, H5N1 Subtype/enzymology/isolation & purification/*physiology
;
Influenza in Birds/*prevention & control/*transmission/virology
;
Oropharynx/virology
;
RNA Replicase/antagonists & inhibitors/genetics/metabolism
;
RNA, Small Interfering/*genetics/metabolism
;
RNA, Viral/analysis/genetics/metabolism
;
Transfection
;
Virus Replication
;
Virus Shedding
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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