Publication Date:
1984-08-10
Description:
Transmissible retroviruses encoding human hypoxanthine phosphoribosyltransferase (HPRT) were used to infect mouse bone marrow cells in vitro, and the infected cells were transplanted into mice. Both active human HPRT-protein and chronic HPRT-virus production were detected in hematopoietic tissue of the mice, showing transfer of the gene. These results indicate the possible use of retroviruses for somatic cell therapy.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Miller, A D -- Eckner, R J -- Jolly, D J -- Friedmann, T -- Verma, I M -- CA 19562/CA/NCI NIH HHS/ -- GM28223/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1984 Aug 10;225(4662):630-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6377498" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bone Marrow/microbiology
;
Bone Marrow Transplantation
;
DNA, Recombinant/metabolism
;
Hematopoietic Stem Cells/microbiology
;
Humans
;
Hypoxanthine Phosphoribosyltransferase/*genetics
;
Isoenzymes/metabolism
;
Lesch-Nyhan Syndrome/genetics/therapy
;
Mice
;
Nucleic Acid Hybridization
;
Rats
;
Retroviridae/enzymology/*genetics
;
Spleen/microbiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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