Abstract
Transmissible retroviruses encoding human hypoxanthine phosphoribosyltransferase (HPRT) were used to infect mouse bone marrow cells in vitro, and the infected cells were transplanted into mice. Both active human HPRT-protein and chronic HPRT-virus production were detected in hematopoietic tissue of the mice, showing transfer of the gene. These results indicate the possible use of retroviruses for somatic cell therapy.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bone Marrow / microbiology
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Bone Marrow Transplantation
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DNA, Recombinant / metabolism
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Hematopoietic Stem Cells / microbiology
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Humans
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Hypoxanthine Phosphoribosyltransferase / genetics*
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Isoenzymes / metabolism
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Lesch-Nyhan Syndrome / genetics
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Lesch-Nyhan Syndrome / therapy
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Mice
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Nucleic Acid Hybridization
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Rats
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Retroviridae / enzymology
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Retroviridae / genetics*
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Spleen / microbiology
Substances
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DNA, Recombinant
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Isoenzymes
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Hypoxanthine Phosphoribosyltransferase