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Determination of sugars by liquid chromatography and amperometric detection with a cuprous oxide modified electrode (1995)

Huang, Xinjian ; Pot, J. J. ; Kok, W. Th.

Springer

Chromatographia 40 (1995), S. 684-689

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Sugars ; Amperometric detection ; Chemically modified electrodes

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A chemically modified electrode was applied as a working electrode for the amperometric detection of sugars in liquid chromatography. A mixture of cuprous oxide and conductive carbon cement was used as electrode material. Cuprous oxide acted as a catalyst for the oxidation of sugars. Cyclic voltammetry was used to examine the oxidation of the sugars at the electrode surface. The stability and sensitivity of a cuprous oxide/conductive carbon cement (20/80 %, w/w) electrode were tested in flow injection analysis experiments. For liquid chromatography, sugars were separated on an ion-exclusion column with 0.01 M H2SO4 as mobile phase. After the separation, 0.2 M NaOH was added post-column and the sugars were determined at 550 mV vs. Ag/AgCl. Calibration was linear in the range of 10−6·10−3 M with limits of detection of approximately 1·10−6M. The application of the modified electrode for the determination of sugars in fruit beverages and dairy products following chromatographic separation is shown.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02315138

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Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.) (1998)

Cho, Y. G. ; McCouch, S. R. ; Kuiper, M. ; [et al.]

Springer

Theoretical and applied genetics 97 (1998), S. 370-380

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ISSN: 1432-2242

Keywords: Key words Amplified fragment length polymorphism (AFLP) ; Simple-sequence length polymorphism (SSLP) ; Restriction fragment length polymorphism (RFLP) ; Rice (Oryza sativa L.) ; Molecular map

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A molecular map of rice consisting of 231 amplified fragment length polymorphisms (AFLPs), 212 restriction fragment length polymorphisms (RFLPs), 86 simple-sequence length polymorphisms (SSLPs), five isozyme loci, and two morphological mutant loci [phenol staining of grain (Ph), semi-dwarf habit (sd-1)] has been constructed using an F11 recombinant inbred (RI) population. The mapping population consisted of 164 RI lines and was developed via single-seed descent from an intercross between the genetically divergent parents Milyang 23 (M) (tongil type) and Gihobyeo (G) ( japonica type). A subset of previously mapped RFLP and SSLP markers were used to construct the map framework. The AFLP markers were derived from ten EcoRI(+2) and MseI(+3) primer combinations. All marker types were well distributed throughout the 12 chromosomes. The integrated map covered 1814 cM, with an average interval size of 3.4 cM. The MG map is a cornerstone of the Korean Rice Genome Research Program (KRGRP) and is being continuously refined through the addition of partially sequenced cDNA markers derived from an immature-seed cDNA library developed in Korea, and microsatellite markers developed at Cornell. The population is also being used for quantitative trait locus (QTL) analysis and as the basis for marker-assisted variety development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220050907

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