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  • 1
    Electronic Resource
    Electronic Resource
    Growth and composition of Escherichia coli subjected to square-wave perturbations in nutrient supply: Effect of varying frequencies (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 21 (1979), S. 1043-1055 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of regularly varying the input nutrient concentration on the growth and composition (elemental and macromolecular) of Escherichia coli ML30 grown in chemostat culture is reported. After each change in substrate concentration a lag between the stimulus and organism response occurred, the magnitude of which was dependent upon the frequency of the perturbations. Both the growth yield and macromolecular composition of the cells were found to vary with the frequency of nutrient cycling: the yield decreased by some 10% as the cycle-times decreased from 6.0 to 0.25 hr. It is suggested that maintaining microorganisms under controlled transient conditions might be useful in increasing yields of specific microbial products (e.g., single-cell protein).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260210609
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  • 2
    Electronic Resource
    Electronic Resource
    Growth and composition of Escherichia coli subjected to square-wave perturbations in nutrient supply: Effect of varying amplitudes (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 22 (1980), S. 1213-1224 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of regular variations in the input limiting nutrient concentration at different cycle amplitudes on the growth and composition (elemental and macromolecular) of Escherichia coli ML30 in chemostat culture is described. The cycle time was maintained constant at 2.0 hr. The basal level of nutrient fed to cultures was also maintained constant but the concentration amplitude was varied over a range from 0.2 to 5.0 g/liter. A lag between the stimulus and organisms response was observed and found to be independent of cycle amplitude over most of the range examined. Increasing the cycle amplitude did not effect the cell yield from glucose or the elemental composition of the organisms. However, large variations in the macromolecular composition of the cells (on a dry weight basis) were found; at 4.0 g/liter amplitude the cells contained some 82% protein. The relevance of this type of reactor operation in large-scale fermentations is discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260220608
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    Paper (German National Licenses)
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