ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
In order to examine the helix-coil transition of water-insoluble polypeptides, without requiring interspersion of charged or polar residues within the sequence, a tri-block copolymer strategy has been developed to determine the helix propensity of amino acids in short (15-residue) peptides. The method is also well suited to evaluate specific interactions that contribute to helix formation. In this approach, measurement is made of the helix content of the central block of tri-block copolymers of the type Lys15-X-Lys15, where X is the 15-residue peptide whose helix content is being investigated. The suitability of tri-block copolymers of this type has been verified experimentally by using the water-soluble peptide (Ala2GlnAla2)3 as the central block. The CD spectrum of the central block in the tri-block copolymer and of Ac-(Ala2GlnAla2)3-NH2 are indistinguishable within experimental error. © 1996 John Wiley & Sons, Inc.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
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