ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Bone marrow derived-stromal cells (BMSCs) are pluriplotent progenitors for avariety of cell types, including osteoblast, chondrocyte, adipocyte, and so on. Stem cell researchhas enormous potential in the future clinical treatment of a wide range of diseases. Tracking stemcell localization, survival, differentiation, and proliferation after transplantation in living subjectsis essential for understanding stem cell biology and physiology. However, we don’t have exactevaluation methods and safeguards for clinical application. In this study, we investigatedtracking BMSCs differentiation, localization, toxicity, and migration in vivo. The fluorescentvector used in our studies did not affect BMSCs viability or their ability to undergo osteogenicand adipogenic differentiation in vitro. During differentiation, EGFP-BMSCs by Oil Red O andAlizarin Red S were stained. EGFP-BMSCs were transplanted into the femoral region inautologous rabbit. After one month, these cells were detectable by confocal microscopy and RTPCR.Transplanted EGFP-BMSCs were not detected another organs (spleen, kidney, liver, andmuscle) in immunohistochemistry and RT-PCR. In organ-function test and cell-toxicityexamination, there is no difference between the before and after EGFP-BMSCs transplantation.we observed that transplanted EGFP-BMSCs were not affected cell-toxicity and migration. Thisresults offer some evaluation methods and safeguards for clinical application using BMSCs. Infurther study, it will be needed to test reproductive and developmental toxicity aftertransplantation and observe migration of EGFP-BMSCs after transplantation
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.137.pdf
Permalink