ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In cartilage tissue engineering, as a cell source, adult stem cells are very attractive forclinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs)have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs areobtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts.Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role incartilage formation. Poly(L-lactide-co-ε-carprolactone) scaffolds have physiological activity,biodegradability, high cell affinity, and mechano-activity. The object of this study is cartilaginoustissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo.Poly(L-lactide-co-ε-carprolactone) copolymers were synthesized from lactide and ε-carprolactonein the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500μmpore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for21days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerasechain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, forexamining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were inducedchondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8weeks.Histological studies showed that implants partially developed cartilaginous tissue within lacunae.And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysisrevealed that implants were positively stained for specific extracellular matrix. These resultsindicate that ASCs and PLCL scaffols could be used to cartilage tissue engineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.385.pdf
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