Keywords: cyclodextrin; cyclodextrin glycosyltransferase; starch; alkalophilic bacillus; Bacillus firmus
Springer Online Journal Archives 1860-2000
Process Engineering, Biotechnology, Nutrition Technology
During screening for cyclodextrin-forming microorganisms, an alkalophilic Bacillus sp, which produced high activity of cyclodextrin glycosyltransferase, was isolated and identified as Bacillus firmus. The crude enzyme transformed starch to mainly β-and γ-cyclodextrin. The purified enzyme had an optimum pH of 7.5–8.5 and its optimum temperature was 65°C, which is the highest optimum temperature as compared to other cyclodextrin glycosyltransferases except that produced by Bacillus amyloliquefaciens.
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