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  • 1
    Publication Date: 2012-06-13
    Description: The purpose of this study was to investigate the protective effects of the mitochondria-targeted antioxidant catalase (MCAT) and lifespan extension in mice that express amyloid beta (Aβ). Using immunoblotting and immunostaining analyses, we measured the production of full-length amyloid precursor protein (APP), soluble APPα, C-terminal fragments CTF99 and CTF83, monomeric and oligomeric Aβ, Aβ deposits and beta site amyloid precursor protein cleaving enzyme 1 (BACE1), in different stages of disease progression in MCAT/AβPP and AβPP mice. Using quantitative reverse transcriptase polymerase chain reaction and immunostaining analyses, we studied the expression of catalase, BACE1, the Alzheimer's disease (AD) markers, synaptophysin, APP, neprilysin, insulin-degrading enzyme and transthyretin in MCAT, AβPP, MCAT/AβPP and wild-type (WT) mice. Using the high pressure liquid chromatography analysis of 8-hydroxy-2-deoxyguanosine, we measured oxidative DNA damage in the cerebral cortical tissues from MCAT, AβPP, MCAT/AβPP and WT mice. We found that the AβPP transgenic mice that carried the human MCAT gene lived 5 months longer than did the AβPP mice. We also found that the overexpression of MCAT in the brain sections from the MCAT/AβPP transgenic mice significantly correlated with a reduction in the levels of full-length APP, CTF99, BACE1, Aβ levels (40 and 42), Aβ deposits and oxidative DNA damage relative to the brain sections from the AβPP mice. Interestingly, we found significantly increased levels of soluble APPα and CTF83 in the MCAT/AβPP mice, relative to the AβPP mice. These data provide direct evidence that oxidative stress plays a primary role in AD etiopathology and that in MCAT mice express Aβ, MCAT prevents abnormal APP processing, reduces Aβ levels and enhances Aβ-degrading enzymes in mice at different ages, corresponding to different stages of disease progression. These findings indicate that mitochondria-targeted molecules may be an effective therapeutic approach to treat patients with AD.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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  • 2
    Publication Date: 2016-06-22
    Description: Journal of Chemical & Engineering Data DOI: 10.1021/acs.jced.6b00135
    Print ISSN: 0021-9568
    Electronic ISSN: 1520-5134
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
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  • 3
    Publication Date: 2016-07-01
    Description: Periodically poled AlN thin films with submicron domain widths were fabricated for nonlinear applications in the UV-VIS region. A procedure utilizing metalorganic chemical vapor deposition growth of AlN in combination with laser interference lithography was developed for making a nanoscale lateral polarity structure (LPS) with domain size down to 600 nm. The Al-polar and N-polar domains were identified by wet etching the periodic LPS in a potassium hydroxide solution and subsequent scanning electron microscopy (SEM) characterization. Fully coalesced and well-defined vertical interfaces between the adjacent domains were established by cross-sectional SEM. AlN LPSs were mechanically polished and surface roughness with a root mean square value of ∼10 nm over a 90  μ m × 90  μ m area was achieved. 3.8  μ m wide and 650 nm thick AlN LPS waveguides were fabricated. The achieved domain sizes, surface roughness, and waveguides are suitable for second harmonic generation in the UVC spectrum.
    Print ISSN: 0003-6951
    Electronic ISSN: 1077-3118
    Topics: Physics
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  • 4
    Publication Date: 2016-06-06
    Description: An abundance analysis is reported of 58-K giants identified by Famaey et al. ( 2005 , A&A, 430, 165) as highly probable members of the Hercules stream selected from stars north of the celestial equator in the Hipparcos catalogue. The giants have compositions spanning the interval [Fe/H] from –0.17 to +0.42 with a mean value of +0.15 and relative elemental abundances [El/Fe] representative of the Galactic thin disc. Selection effects may have biased the selection from the Hipparcos catalogue against the selection of metal-poor stars. Our reconsideration of the recent extensive survey by Bensby et al. of FG dwarfs, including metal-poor stars, provides a [Fe/H] distribution for the Hercules stream, which is similar to that from the 58 giants. It appears that the stream is dominated by metal-rich stars from the thin disc. We discuss suggestions in the literature that the stream includes metal-poor stars from the thick disc.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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  • 5
    Publication Date: 2014-03-05
    Description: Wireworms, the larvae stage of click beetles (family, Elateridae), are serious soil dwelling pests of small grain, corn, sugar beet, and potato crops globally. Since the 1950s, conventional insecticides such as lindane provided effective and inexpensive protection from wireworms, and little integrated pest management research (IPM) was conducted. The removal of these products from the agricultural market, particularly Lindane, has resulted in increasing levels of wireworm damage to small grain, corn, and potato crops. The wireworm damage has become an increasing problem for growers, so the demand for a meaningful risk assessment and useful methods to restrict damage is increasing. However, due to the cryptic habitat of the wireworms, pest control is very difficult and leads to unsatisfying results. The prospective appropriateness of sex pheromone traps for employing management strategies against wireworm’s populations was first suggested with experimentation in Hungary and Italy. Simultaneously, considerable work has been done on the identification and use of pheromone traps to monitor population of click beetles. The work has been mostly done in European and former Soviet Union countries. For this paper, we reviewed what work has been done in monitoring the click beetle which was considered as pests and how the pheromones can be used in IPM to monitor and control wireworms/click beetles. Also, the possibilities of using the pheromone-baited traps for mating disruption and control tested in the fields were summarized.
    Electronic ISSN: 2090-7524
    Topics: Geosciences , Physics
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  • 6
    Publication Date: 2014-03-05
    Description: Wireworms, the larvae stage of click beetles (family, Elateridae), are serious soil dwelling pests of small grain, corn, sugar beet, and potato crops globally. Since the 1950s, conventional insecticides such as lindane provided effective and inexpensive protection from wireworms, and little integrated pest management research (IPM) was conducted. The removal of these products from the agricultural market, particularly Lindane, has resulted in increasing levels of wireworm damage to small grain, corn, and potato crops. The wireworm damage has become an increasing problem for growers, so the demand for a meaningful risk assessment and useful methods to restrict damage is increasing. However, due to the cryptic habitat of the wireworms, pest control is very difficult and leads to unsatisfying results. The prospective appropriateness of sex pheromone traps for employing management strategies against wireworm’s populations was first suggested with experimentation in Hungary and Italy. Simultaneously, considerable work has been done on the identification and use of pheromone traps to monitor population of click beetles. The work has been mostly done in European and former Soviet Union countries. For this paper, we reviewed what work has been done in monitoring the click beetle which was considered as pests and how the pheromones can be used in IPM to monitor and control wireworms/click beetles. Also, the possibilities of using the pheromone-baited traps for mating disruption and control tested in the fields were summarized.
    Electronic ISSN: 2090-8989
    Topics: Geosciences , Physics
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  • 7
    Publication Date: 2014-02-26
    Description: The B-myb (MYBL2) gene is a member of the MYB family of transcription factors and is involved in cell cycle regulation, DNA replication, and maintenance of genomic integrity. However, its function during adult development and hematopoiesis is unknown. We show here that conditional inactivation of B-myb in vivo results in...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 8
    Publication Date: 1998-11-13
    Description: The ectodomains of numerous proteins are released from cells by proteolysis to yield soluble intercellular regulators. The responsible protease, tumor necrosis factor-alpha converting enzyme (TACE), has been identified only in the case when tumor necrosis factor-alpha (TNFalpha) is released. Analyses of cells lacking this metalloproteinase-disintegrin revealed an expanded role for TACE in the processing of other cell surface proteins, including a TNF receptor, the L-selectin adhesion molecule, and transforming growth factor-alpha (TGFalpha). The phenotype of mice lacking TACE suggests an essential role for soluble TGFalpha in normal development and emphasizes the importance of protein ectodomain shedding in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Peschon, J J -- Slack, J L -- Reddy, P -- Stocking, K L -- Sunnarborg, S W -- Lee, D C -- Russell, W E -- Castner, B J -- Johnson, R S -- Fitzner, J N -- Boyce, R W -- Nelson, N -- Kozlosky, C J -- Wolfson, M F -- Rauch, C T -- Cerretti, D P -- Paxton, R J -- March, C J -- Black, R A -- CA43793/CA/NCI NIH HHS/ -- DK53804/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1998 Nov 13;282(5392):1281-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Immunex Corporation, Seattle, WA 98101, USA. peschon@immunex.com〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9812885" target="_blank"〉PubMed〈/a〉
    Keywords: ADAM Proteins ; Amino Acid Sequence ; Animals ; Catalytic Domain ; Cell Membrane/*metabolism ; Cells, Cultured ; Crosses, Genetic ; *Embryonic and Fetal Development ; L-Selectin/metabolism ; Ligands ; Membrane Proteins/*metabolism ; Metalloendopeptidases/chemistry/genetics/*metabolism ; Mice ; Mice, Inbred C57BL ; Molecular Sequence Data ; Mutation ; Phenotype ; Protein Processing, Post-Translational ; Receptors, Tumor Necrosis Factor/metabolism ; Transforming Growth Factor alpha/metabolism ; Tumor Necrosis Factor-alpha/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
  • 10
    Publication Date: 2015-12-08
    Description: Radiative transfer of energy at the nanometre length scale is of great importance to a variety of technologies including heat-assisted magnetic recording, near-field thermophotovoltaics and lithography. Although experimental advances have enabled elucidation of near-field radiative heat transfer in gaps as small as 20-30 nanometres (refs 4-6), quantitative analysis in the extreme near field (less than 10 nanometres) has been greatly limited by experimental challenges. Moreover, the results of pioneering measurements differed from theoretical predictions by orders of magnitude. Here we use custom-fabricated scanning probes with embedded thermocouples, in conjunction with new microdevices capable of periodic temperature modulation, to measure radiative heat transfer down to gaps as small as two nanometres. For our experiments we deposited suitably chosen metal or dielectric layers on the scanning probes and microdevices, enabling direct study of extreme near-field radiation between silica-silica, silicon nitride-silicon nitride and gold-gold surfaces to reveal marked, gap-size-dependent enhancements of radiative heat transfer. Furthermore, our state-of-the-art calculations of radiative heat transfer, performed within the theoretical framework of fluctuational electrodynamics, are in excellent agreement with our experimental results, providing unambiguous evidence that confirms the validity of this theory for modelling radiative heat transfer in gaps as small as a few nanometres. This work lays the foundations required for the rational design of novel technologies that leverage nanoscale radiative heat transfer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kim, Kyeongtae -- Song, Bai -- Fernandez-Hurtado, Victor -- Lee, Woochul -- Jeong, Wonho -- Cui, Longji -- Thompson, Dakotah -- Feist, Johannes -- Reid, M T Homer -- Garcia-Vidal, Francisco J -- Cuevas, Juan Carlos -- Meyhofer, Edgar -- Reddy, Pramod -- England -- Nature. 2015 Dec 17;528(7582):387-91. doi: 10.1038/nature16070. Epub 2015 Dec 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA. ; Departamento de Fisica Teorica de la Materia Condensada and Condensed Matter Physics Center (IFIMAC), Universidad Autonoma de Madrid, Madrid 28049, Spain. ; Department of Mathematics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. ; Donostia International Physics Center (DIPC), Donostia/San Sebastian 20018, Spain. ; Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26641312" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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