Publication Date:
2002-05-11
Description:
Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms (SNPs), inserted or deleted sequences, and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences among different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Read, Timothy D -- Salzberg, Steven L -- Pop, Mihai -- Shumway, Martin -- Umayam, Lowell -- Jiang, Lingxia -- Holtzapple, Erik -- Busch, Joseph D -- Smith, Kimothy L -- Schupp, James M -- Solomon, Daniel -- Keim, Paul -- Fraser, Claire M -- R01-LM06845/LM/NLM NIH HHS/ -- New York, N.Y. -- Science. 2002 Jun 14;296(5575):2028-33. Epub 2002 May 9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA., Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12004073" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Anthrax/microbiology
;
Bacillus anthracis/classification/*genetics/isolation &
;
purification/pathogenicity
;
Bacterial Typing Techniques
;
Base Sequence
;
Bioterrorism
;
Chromosome Inversion
;
Computational Biology
;
Disease Outbreaks
;
Genetic Markers
;
*Genetic Variation
;
*Genome, Bacterial
;
Genomics
;
Humans
;
Minisatellite Repeats
;
Molecular Sequence Data
;
Mutation
;
Phenotype
;
Phylogeny
;
Plasmids
;
*Polymorphism, Single Nucleotide
;
Recombination, Genetic
;
Repetitive Sequences, Nucleic Acid
;
*Sequence Analysis, DNA
;
Sequence Deletion
;
Species Specificity
;
Transposases/genetics
;
Virulence/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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