ISSN:
1432-0800
Source:
Springer Online Journal Archives 1860-2000
Topics:
Energy, Environment Protection, Nuclear Power Engineering
,
Medicine
Notes:
Summary Monolayer cultures of HeLa cells were exposed to an insecticidal chemical, chlorphenamidine, and its effect on nucleic acid and protein synthesis was measured. No significant differences among the effects of the chemical on DNA, RNA, and protein synthesis were observed. The rates of the synthesis of macromolecules were inhibited by 40 to 50 percent at a concentration of 10−3 M. At lower dose levels (10−4 M and 10−5 M), the synthesis of macromolecules was virtually unaffected. The cytotoxicity of chlorphenamidine, its potential metabolites, and an analog, 5-chloro-2-nitrotoluene, was studied and the growth inhibition (ID50) based on inhibition of cell protein synthesis was determined. Chlorphenamidine and its two sequential metabolites, demethylchlorphenamidine andN-formyl-4-chloro-o-toluidine, had almost equal toxicity. The proposed final metabolite, 4-chloro-2-methylaniline was the least toxic compound and a chlorphenamidine analog, 5-chloro-2-nitrotoluene, was most toxic. These results closely correlated with the comparative inhibitory activity of these compounds for RNA synthesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01684602
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