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  • 1
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on callus growth and protoplast plating efficiency of Solanum dulcamara (1991)
    Springer
    Plant cell reports 10 (1991), S. 52-54 
    Details
    ISSN: 1432-203X
    Keywords: Pluronic F-68 ; Callus growth ; Protoplasts ; Solanum dulcamara
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the non-ionic surfactant, Pluronic F-68, on the growth of callus and protoplasts from Solanum dulcamara L. have been studied. Growth of callus was stimulated by addition of 0.1% (w/v) commercial grade Pluronic to culture medium, whereas lower concentrations (0.01% w/v) had no corresponding effect. In contrast, higher concentrations (1.0% w/v) of Pluronic inhibited callus growth. The mean plating efficiency of protoplasts grown at different densities (15 days after plating) was increased up to 26% following culture with 0.1% (w/v) Pluronic, while 0.01% (w/v) Pluronic was ineffective. Mean protoplast plating efficiency decreased by up to 32% following culture with 1.0% (w/v) Pluronic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233033
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts (1988)
    Springer
    Plant cell reports 7 (1988), S. 379-384 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic rice plants have been regenerated by somatic embryogenesis from cell suspension derived protoplasts electroporated with plasmid carrying the NPTII gene under the control of the 35S promoter from cauliflower mosaic virus. Heat shock of protoplasts prior to electroporation maximised the throughput of kanamycin resistant colonies. Omission of kanamycin from the medium for plant regeneration was essential for the recovery of transgenic rice plants carrying the NPTII gene. This report of the production of kanamycin resistant transgenic rice plants establishes the use of protoplasts for rice genetic engineering.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269517
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of a chimaeric kanamycin resistance gene introduced into the wild soybeanGlycine canescens using a cointegrate Ri plasmid vector (1989)
    Springer
    Plant cell reports 8 (1989), S. 33-36 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedling hypocotyl explants ofGlycine canescens were inoculated withAgrobacterium rhizogenes carrying a chimaeric NPTII gene cointegrated into the TL-DNA of pRiA4. Transformed roots produced shoots on B5 based medium with 10.0 mgl−1 BAP, 0.05 mgl−1 IBA and 50 μgml−1 kanamycin. Cultured roots and regenerated plants expressed NPTII enzyme activity which was correlated with the presence of Ri TL-DNA and the structural sequence of the NPTII gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00735773
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  • 4
    Electronic Resource
    Electronic Resource
    Interspecific somatic hybrids ofRudbeckia hirta andR. Laciniata (Compositae) (1990)
    Springer
    Plant cell reports 8 (1990), S. 517-520 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interspecific somatic hybrid plants betweenRudbeckia hirta cv. Marmalade andR.laciniata cv. Irish Eyes were regenerated following the electro-fusion of mesophyll protoplasts ofR.hirta with callus protoplasts ofR.laciniata. A hybrid selection scheme was based on the fact that plant regeneration, from parental protoplasts ofR.hirta, was via shoot regeneration of callus, and only via rhizogenesis forR.laciniata. The other half of the selection strategy was based on the presence of anthocyanin-pigmented roots; a characteristic of theR.hirta parent only. Somatic hybrids were regenerated, via rhizogenesis, alongside normalR.laciniata but were distinguished by the presence of pigmented roots (a feature ofR.hirta). Hybrid plants had a floral morphology that was intermediate as compared to that of the two parents, with an expected somatic chromosome number of 2n=(2x+4x)=74. Pollen viability though was low. Esterase and peroxidase isozyme profiles confirmed the hybrid nature of the regenerated plants with pigmented roots, whilst chloroplast DNA restriction analysis showed that these hybrids had aR.laciniata chloroplast DNA. This demonstration of somatic hybridisation not only opens up the possibility of incorporating novel traits between such ornamentalCompositae species, but provides a selection strategy based on rhizogenesis as the route to plant regeneration coupled with heritable pigmentation production of roots as a confirmatory hybrid marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00820199
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  • 5
    Electronic Resource
    Electronic Resource
    Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene (1987)
    Springer
    Plant cell reports 6 (1987), S. 297-301 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 107 following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml−1 kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3′)II activity, and contained DNA that hybridised to a probe with the APH(3′)II gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272003
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  • 6
    Electronic Resource
    Electronic Resource
    Production of kanamycin resistant rice tissues following DNA uptake into protoplasts (1988)
    Springer
    Plant cell reports 7 (1988), S. 421-425 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA that hybridised to a 1.0 Kb BamHI fragment of pCaMVNEO carrying the NPTII gene. Expression of the transformation frequency in relative terms (number of kanamycin resistant colonies compared to the number of colonies on kanamycin free medium) gave frequencies of 26.1%, 8.5% and 2.9% following electroporation, PEG and PEG with electroporation respectively. In absolute terms (number of kanamycin resistant colonies compared to the number of protoplasts plated) these represent frequencies of 19.9×10−5, 9.0×10−5 and 2.7×10−5 for the three procedures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269528
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  • 7
    Electronic Resource
    Electronic Resource
    Roots induced on cucumber cotyledons by the agropine Ri plasmid TR-DNA exhibit the transformed phenotype (1991)
    Springer
    Plant cell reports 9 (1991), S. 647-650 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cucumber explants were transformed by Agrabacterium strains carrying Ri plasmids with functional TL and TR-DNAs, and by strains whose pRi had an intact TR-DNA but a disarmed TL-DNA lacking open reading frames (ORFs) 3 to 9, 10 (rol A), 11 (rol B), 12 (rol C), 13, 14, 15 (rol D), 16 and 17. Roots induced by all strains exhibited extensive root hair formation under axenic conditions, synthesised opines, and contained TR-specific DNA. These results confirm that the TR-DNA of an agropine Ri plasmid is able to elicit the transformed root phenotype in this plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231808
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  • 8
    Electronic Resource
    Electronic Resource
    Small mitochondrial DNA molecules of wild abortive cytoplasm in rice are not necessarily associated with CMS (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 617-619 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; Mitochondrial DNA ; Plasmid-like molecules ; Wild abortive cytoplasm ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA was isolated from leaf tissue of both the cytoplasmic male sterile line of Indica rice variety V41, which carries wild abortive (WA) cytoplasm, and from the corresponding maintainer line. In addition to the main mitochondrial DNA, four small plasmid-like DNA molecules were detected in both the male sterile and fertile lines. Restriction analysis of total mitochondrial DNA from the male sterile and fertile lines showed DNA fragments unique to each. Our findings suggest that the four small mitochondrial DNA (mtDNA) molecules are conserved when WA cytoplasm is transferred into different nuclear backgrounds. However, there is no simple correlation between the presence/ absence of small mitochondrial DNA molecules and the expression of WA cytoplasmic male sterility (CMS).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261232
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  • 9
    Electronic Resource
    Electronic Resource
    Stability of mitochondrial DNA in tissue-cultured cells of rice (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 342-346 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Rice ; S2 cytoplasmic male sterility ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction analysis of mitochondrial (mt) DNA from 3-month-old callus cultures of the cytoplasmic male sterile rice, V41A, which contains S2 or “wild abortive” cytoplasm, and its fertile maintainer, V41B, showed the same BamHI restriction profiles as mtDNA from the corresponding leaf material. Similarly, mtDNA of rice (var. Taipei 309) from leaves, a 2-month-old cell suspension (T3MS2/A), a totipotent suspension (T3MS) and a 19-month-old suspension, which had lost its protoplast regeneration ability (LB3), showed indistinguishable BamHI restriction profiles. However, clear differences in mtDNA restriction profiles were observed between LB3 and a 30-month-old suspension culture of Taipei 309 (LB1), which appeared to reflect substantial changes in the relative abundance of specific DNA sequences. Hybridisation of a maizecoxII gene probe to blots of restricted mtDNA confirmed that, while the relative abundance of certain mtDNA sequences was preserved during long-term tissue culture of rice, major changes in abundance were observed with other sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01186077
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of Pluronic F-68 on shoot regeneration from cultured jute cotyledons and on growth of transformed roots (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 133-140 
    Details
    ISSN: 1573-5044
    Keywords: Corchorus capsularis ; medium supplement ; necrosis ; organogenesis ; suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects have been studied of the non-ionic surfactant, Pluronic F-68, on the growth in culture of jute (Corchorus capsularis L.) cotyledons with attached petioles, cotyledon explants and transformed roots. Supplementation of culture medium with 0.001–0.5% (w/v) of either commercial grade Pluronic F-68 or a purified fraction prepared by passage through silica gel, stimulated shoot production from the petioles of C. capsularis var. D154 and C134 cotyledons. This effect was most marked in C134, because of the failure of control cotyledons to produce shoots in the absence of Pluronic. Plants regenerated from Pluronic-treated cotyledons were morphologically normal. Growth of transformed roots of C. capsularis var. D154 was stimulated in medium supplemented with commercial grade or purified Pluronic F-68, with maximum increases in both fresh and dry weights with 0.1% (w/v) of the surfactant. Roots cultured in the presence of Pluronic F-68 could be maintained without sub-culture for up to 70 days, whereas roots cultured in the absence of Pluronic required subculture every 7 days, to prevent necrosis. Transformed roots also produced callus in the presence of 0.001–1.0% (w/v) of either commercial grade or purified Pluronic. The biotechnological implications of these results are discussed in relation to the potential value of non-ionic surfactants as growth-stimulating additives to plant culture media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036093
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