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  • 1
    Electronic Resource
    Electronic Resource
    Stability of mitochondrial DNA in tissue-cultured cells of rice (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 342-346 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Rice ; S2 cytoplasmic male sterility ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction analysis of mitochondrial (mt) DNA from 3-month-old callus cultures of the cytoplasmic male sterile rice, V41A, which contains S2 or “wild abortive” cytoplasm, and its fertile maintainer, V41B, showed the same BamHI restriction profiles as mtDNA from the corresponding leaf material. Similarly, mtDNA of rice (var. Taipei 309) from leaves, a 2-month-old cell suspension (T3MS2/A), a totipotent suspension (T3MS) and a 19-month-old suspension, which had lost its protoplast regeneration ability (LB3), showed indistinguishable BamHI restriction profiles. However, clear differences in mtDNA restriction profiles were observed between LB3 and a 30-month-old suspension culture of Taipei 309 (LB1), which appeared to reflect substantial changes in the relative abundance of specific DNA sequences. Hybridisation of a maizecoxII gene probe to blots of restricted mtDNA confirmed that, while the relative abundance of certain mtDNA sequences was preserved during long-term tissue culture of rice, major changes in abundance were observed with other sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01186077
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  • 2
    Electronic Resource
    Electronic Resource
    Flow cytometric quantification of electroporation-mediated uptake of macromolecules into plant protoplasts (1995)
    Springer
    Protoplasma 186 (1995), S. 50-56 
    Details
    ISSN: 1615-6102
    Keywords: Electroporation ; Flow cytometry ; Macromolecules ; Protoplasts ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Flow cytometry was used to provide a rapid and accurate assessment of electroporation-induced uptake of macromolecules into plant protoplasts. Rice protoplasts were electroporated in the presence of fluorescein isothiocyanate-conjugated dextran (FITC-dextran). After washing, the protoplasts were resuspended in a solution containing propidium iodide which intercalates with DNA, but which is excluded by an intact plasma membrane. Electroporation in the presence of FITC-dextran gave rise to populations of protoplasts that fluoresced green or yellow due to the presence of non-conjugated FITC. Non-viable protoplasts fluoresced red because of their inability to exclude propidium iodide molecules. Flow cytometry was used to resolve and quantify these protoplast populations and thus identify optimal conditions for macromolecule uptake. A direct relationship was observed between FITC-dextran uptake and transient gene expression following plasmid uptake. Thus, simultaneous electroporation of protoplasts with foreign DNA and FITC-dextran followed by fluorescence activated cell sorting may permit partial selection of transformed cells and so reduce the need for a selectable marker.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276935
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    Paper (German National Licenses)
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