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  • 1
    Electronic Resource
    Electronic Resource
    DNA Methylation in Escherichia Coli (1987)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 21 (1987), S. 113-131 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.21.120187.000553
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of the Escherichia coli dam gene (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. PI and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2, was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2, and the aroB gene. This 16 kDa open reading frame has been Identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the σ70 subunit of the RNA polymerase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb01356.x
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  • 3
    Electronic Resource
    Electronic Resource
    Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single-strand binding protein (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 4 (1990), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Single-stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates were deletions of apparently random length and substitutions (deletion/insertions). The intergenic deletions as well as the transition and frameshift mutations were identical to those previously isolated from mismatch repair-defective cells in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1990.tb00541.x
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  • 4
    Electronic Resource
    Electronic Resource
    Novel growth rate control of dam gene expression in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 12 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Transcription of the dam gene in Escherichia coli is growth rate regulated by a mechanism distinct from that used for ribosomal RNA gene promoters. Single-copy operon fusions to lacZ indicated that the major promoter, P2, is responsible for most or all of the growth rate dependence. Promoter P2 is a typical σ70 promoter with 18bp spacing between the -10 and -35 hexamers. Primer extension analysis was used to show that there was no inhibition of transcription from promoter P2 in cells induced for the stringent response. Beta-galactosidase specific activity from a single-copy dam::lacZ fusion was unaffected by either excess rrnB RNA or the level of Fis protein. Thus growth rate control of dam gene expression differs from that of the rRNA and tRNA genes by its lack of response to stringent control, ribosomal feedback and enhanced transcription by Fis protein. We devised a procedure for selection of mutant cells in which dam gene expression was unregulated. One such mutant (cde-4), obtained by miniTn 10 insertion, showed the same level of β-galactosidase activity at all growth rates tested, in contrast, growth rate-dependent expression of the rrnB gene was unaffected by cde-4 confirming the different modes of regulation. The cde-4::mint Tn 10 insertion is located close to kilobase 670 on the physical map in or near the lipB gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb01050.x
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  • 5
    Electronic Resource
    Electronic Resource
    Role of plasmid multimers in mutation to tetracyline resistance (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: As an additional system for analysing mutations that appear to be specifically induced or directed, we have used a plasmid that contains the mnt repressor gene inserted as an operon fusion with the tet gene of the plasmid pBR322. Thus, the mnt gene product acts as a negative transcriptional regulator of tet gene expression. Mutations inactivating the Mnt repressor are recessive while those destroying operator recognition (Oc) are dominant in conferring tetracycline resistance on the host. When resistance mutations were isolated on plates with high levels of tetracycline they were preferentially mnt- and the plasmids were monomers. Pre-exposure to low concentrations increased the frequency of resistant mutants by 100- to 1000-fold, and the mutations were now mostly Oc, located on one unit of a plasmid multimer. Recessive repressor mutations on one unit would not have been selected. We suggest that the high frequency of mutation in tandem multimeric plasmids may be caused by the formation of single-stranded and hence highly mutable regions by homologous pairing out of register. The role of tetracycline in promoting mutations is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb02100.x
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  • 6
    Electronic Resource
    Electronic Resource
    Mismatch correction at O 6 -methylguanine residues in E. coli DNA (1982)
    [s.l.] : Nature Publishing Group
    Nature 296 (1982), S. 868-869 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The major repair pathway for the promutagenic base m6G in E. coli is via a methyltransferase induced as part of the adaptive response to alky la ting agents7'9. The adaptive response is induced by exposure to sublethal concentrations of alkylating agents and renders cells resistant to the lethal ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/296868a0
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  • 7
    Electronic Resource
    Electronic Resource
    Location of DNA methylation genes on the Escherichia coli K-12 genetic map (1973)
    Springer
    Molecular genetics and genomics 127 (1973), S. 47-55 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes responsible for DNA adenine methylation (dam) and DNA cytosine methylation (dcm) have been mapped on the E. coli K-12 genetic map. The dam gene is situated at min 65 and the gene order cysG-(trpS, dam)-aro B inferred. The dcm gene is located at min 37.5 and the gene order is supD-dcm-flaA1. In F′ merodiploids, the dam and dcm alleles are recessive.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267782
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  • 8
    Electronic Resource
    Electronic Resource
    DNA methylation influences trpR promoter activity in Escherichia coli K-12 (1985)
    Springer
    Molecular genetics and genomics 200 (1985), S. 185-186 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Methylation of adenine in the GATC-sequence of the-35 region of the trpR promoter decreases activity by 2–3 fold.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00383334
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  • 9
    Electronic Resource
    Electronic Resource
    Hyper-recombination in dam mutants of Escherichia coli K-12 (1976)
    Springer
    Molecular genetics and genomics 149 (1976), S. 273-277 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30–200 times higher than the isogenic dam + strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduction in linkage of proximal unselected markers in transconjugants and increases the recombination frequency between a pair of closely linked markers. It is concluded that dam mutations confer a hyperrecombination phenotype to the cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268528
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  • 10
    Electronic Resource
    Electronic Resource
    Induction of damage inducible (SOS) repair in dam mutants of Escherichia coli exposed to 2-aminopurine (1984)
    Springer
    Molecular genetics and genomics 194 (1984), S. 539-540 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 2-Aminopurine induces damage inducible (SOS) repair in an Escherichia coli dam-4 strain but not in a dam-4 mutS456 derivative or in dam + bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425572
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