ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Energy conservation during nitrate respiration in Paracoccus denitrificans (1977)
    Springer
    Archives of microbiology 112 (1977), S. 17-23 
    Details
    ISSN: 1432-072X
    Keywords: Oxidative phosphorylation ; Proton translocation ; Continuous culture ; Maintenance energy ; Growth yield ; Nitrate respiration ; Paracoccus denitrificans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract P/2e ratios were calculated from anaerobic chemostat cultures of Paracoccus denitrificans with nitrogenous oxides as electron acceptor. P/2e ratios were calculated, using the Y ATP max values determined for aerobic cultures. When succinate was the carbon and energy source the average P/2e values of the sulphate-and succinate-limited cultures with nitrate as electron acceptor were 0.5 and 0.7, respectively, and of the nitrite-limited culture 0.9. With gluconate as carbon and energy source the average P/2e values of the gluconate-limited with nitrate as electron acceptor and nitrate limited cultures were 0.9 and 1.1, respectively. →H+/O ratios measured in cells obtained from sulphate-, succinate, nitrite-, gluconate-and nitratelimited cultures yielded respective average values of 3.4, 4.5, 3.5, 4.8 and 6.2 for endogenous substrates. From our data we conclude that sulphate-and nitritelimitation causes the loss of site I phosphorylation. Nitrite has no influence on the maximum growth yield on ATP. We propose that metabolism in heterotrophically grown cells of Paracoccus dentrificans is regulated on the level of phosphorylation in the site I region of the electron transport chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446649
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Energy conservation during aerobic growth in Paracoccus denitrificans (1977)
    Springer
    Archives of microbiology 112 (1977), S. 25-34 
    Details
    ISSN: 1432-072X
    Keywords: Oxidative phosphorylation ; Proton translocation ; Continuous culture ; Sulphate limitation ; Iron limitation ; Growth yield ; Maintenance energy ; Paracoccus denitrificans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paracoccus denitrificans was aerobically grown in chemostat culture with succinate or gluconate as carbon source. Due to the presence of two phosphorylation sites in the respiratory chain and the absence of branching, theoretical P/O ratios of 1.71 and 1.82 were calculated for cells growing respectively with succinate and gluconate as carbon source. Using these data, 95% confidence intervals for the P/O ratio were determined, via a mathematical model, at 0.91–1.15 and 1.00–1.37 for sulphate-limited cultures, with respectively succinate and gluconate as carbon source. These results and measurements of P/O ratios in membrane particles and of proton translocation in whole cells have led to the conclusion that site I phosphorylation is affected under sulphate-limited conditions. Under conditions of carbon source-limitation the endogenous →H+/O ratio is about 7–8. Average values of 3.40 and 4.78 were respectively found for sulphate-limited succinate- and gluconate grown cells. For starved cells, oxidizing succinate as exogenous substrate, the →H+/O ratios were determined at about 3–4, independent of the growth limiting factor. It is concluded that the number of protons ejected per pair of electrons per energy-conserving site (→H+/site ratio) is about 3–4, instead of 2 as postulated by the chemiosmotic hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446650
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Effects induced by rotenone during aerobic growth ofParacoccus denitrificans in continuous culture (1978)
    Springer
    Archives of microbiology 119 (1978), S. 119-127 
    Details
    ISSN: 1432-072X
    Keywords: NADH dehydrogenase ; Energy conservation ; Electron transport ; Rotenone sensitivity ; Iron-sulfur centers ; Paracoccus denitrificans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paracoccus denitrificans was grown aerobically in chemostat culture in the presence of rotenone. After 6 to 10 generation times, cells showed an oxygen uptake which was completely rotenone-insensitive after removal of rotenone by washing with bovine serum albumin containing medium. The →H+/O ratio of these cells for endogenous substrates decreased from about 7.50 to 3.95. The latter ratio was similar to the value obtained for starved cells oxidizing exogenous succinate, indicating that site I phosphorylation was absent in these rotenone-insensitive cells. Membrane particles prepared from these cells showed an 80% decrease in activity of reduced nicotinamide adenine dinucleotide oxidase and reduced nicotinamide adenine dinucleotide-ferricyanide oxidoreductase, while also the kinetic behaviour of the reduced nicotinamide adenine dinucleotide dehydrogenase in the reduced nicotinamide adenine dinucleotide-ferricyanide oxidoreductase assay was changed. Moreover the reduced nicotinamide adenine dinucleotide oxidase activity was practically rotenone-insensitive. Electron paramagnetic resonance spectroscopy on membrane particles from rotenone-insensitive cells at 15 K revealed that the resonance lines atg z ≈ 2.05 andg y ≈g x ≈ 1.92 arising from iron-sulfur center 2 were undetectable. The intensities of the other electron paramagnetic resonance signals originating from reduced nicotinamide adenine dinucleotide dehydrogenase linked iron-sulfur centers were only slightly diminished. These observations confirm our previous suggestion that site I phosphorylation, rotenone sensitivity and the presence of iron-sulfur center 2 are correlated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00964262
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    New Developments in the Synthesis of Natural and Unnatural Amino Acids (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 672 (1992), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Notes: Amino acids play an important role in biochemistry and chemistry. They are the building blocks of proteins and play an essential role in the regulation of the metabolism of living organisms.In general, it can be stated that microbial processes (fermentation) are the industrial production methods of choice for large-scale production of naturally occuring proteinogenic L-α-H-amino acids, while for the production of synthetic D- and/or L-α-H-amino acids, several other methods are highly competitive.At DSM, several routes, i.e., (chemoenzymatic) synthesis, towards L-α-H and D-α-H-amino acids have been elaborated since the midseventies.A general process for the synthesis of natural as well as synthetic optically pure amino acids has been developed, using an enzymatic kinetic resolution step on racemic amino acid amides as the key step. In this case, both enantiomers of the α-H-amino acids are prepared in one single step. This process has been commercialized since 1988.More recent developments using L- or D-amino peptidases in combination with amino acid amide racemases and an asymmetric transformation concept are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1992.tb35665.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of sulphur dioxide on leaf photosynthesis: the role of temperature and humidity (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 80 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of temperature and humidity on SO2–induced photosynthetic depression was determined in gas exchange experiments with leaves of Vicia faba, L. Stomatal behaviour was sensitive to humidity resulting in higher uptake rates of SO2 and stronger reductions of photosynthesis at low VPD (vapour pressure deficit). After a fumigation period of 2 h, when the photosynthetic rate had stabilized, photosynthesis of leaves exposed to SO2 at 8°C was reduced much more than at 18°C at the same rate of SO2 uptake. Data analysis with a mechanistic model revealed that this effect was due to the slower rate of S(IV) oxidation at lower temperatures, resulting in higher accumulation of S(IV) and thus stronger reduction of photosynthesis. These results were confirmed by experimental analyses of the S(IV) concentration in leaves following fumigation, which showed that more S(IV) accumulated in leaves exposed at a lower temperature. This may explain the high sensitivity of plants exposed to SO2 under winter conditions, when both VPD and temperature are low.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05693.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase (1991)
    Springer
    Applied microbiology and biotechnology 35 (1991), S. 216-221 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Favourable reaction conditions for the enzymatic production of 1-kestose by sucrose-1F-fructosyltransferase, SFT (EC 2.4.1.99) from Aspergillus phoenicis CBS 294.80 mycelium were established. The intracellular enzyme SFT works best at 60°C, exhibits a relatively high thermostability and possesses an alkaline pH optimum. An invertase also present in the mycelium of A. phoenicis possesses an acidic pH optimum. Consequently, around pH 8.0 sucrose is converted mainly to 1-kestose and nystose while fructose is only formed in relatively small amounts. Under optimal conditions (55° C, pH 8.0 and an initial sucrose concentration of 750 g 1-1) a yield of about 300 g 1-kestose per 1.01 reaction mixture could be achieved after 8 h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184689
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    An enzymatic process for the production of fructose from inulin (1983)
    Springer
    Antonie van Leeuwenhoek 49 (1983), S. 88-88 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00457890
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Anaerobic respiration and energy conservation in Paracoccus denitrificans. Functioning of iron-sulfur centers, the uncoupling action of nitrite and stoichiometries for proton pumping (1979)
    Springer
    Antonie van Leeuwenhoek 45 (1979), S. 329-330 
    Details
    ISSN: 1572-9699
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418606
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Quantification of microsclerotia ofVerticillium dahliae in plant material by image analysis (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 705-709 
    Details
    ISSN: 1573-8469
    Keywords: image analysis ; microsclerotia ; potato ; Solanum tuberosum ; Verticillium dahliae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A procedure for the quantification of microsclerotia ofVerticillium dahliae with an image analysis system was compared with counting by eye. Colonised potato plant material was used from plants grown in pathogen-free soil in a greenhouse and from twelve crops (including four potato cultivars) grown outdoors in pots filled with pathogen-free soil under natural conditions. The values obtained from the potato material from the greenhouse were comparable for both methods. Variation in the results mainly resulted from sampling errors. The numbers of microsclerotia in plants grown outdoors were overestimated by image analysis for most crops. The source of the error was related to the presence of plant and soil particles that did not discolour during boiling of the samples in sodium hydroxide. Image analysis was a suitable and reliable method for assessing the number of microsclerotia only in potato haulm samples from plants grown in pathogen-free soil in the greenhouse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874876
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Vanadium containing bromoperoxidase: An example of an oxidoreductase with high operational stability in aqueous and organic mediaSend all correspondence to Ms. G. E. E. van Noppen, F.I.L., Publications Secretary, Laoratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD Amsterdam, The Netherlands. (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 607-610 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The conversion is described of phenolsulphonephtalein (phenol red) to 3,3′,5,5′-tetrabromophenolsulphonephthalein (bromophenol blue) by bromoper-oxidase from the brown alga Ascophyllum nodosum. This reaction provides a convenient assay for the detection of bromoperoxidase activity in vitro. Bromoperoxidase was shown to be stable under turnover conditions for three weeks at room temperature, catalyzing the bromination of phenol red into bromophenol blue. When stored at room temperature in organic sol vents such as acetone, methanol, ethanol [present up to 60% (v/v)], and 1-propanol [40% (v/v)], bromoperoxidase was stable for more than one month. As far as we know this is the first example of an oxidoreductase which displays such great stability. This enhances the applicability of the enzyme in organic synthesis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260300504
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...