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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 110 (1976), S. 195-206 
    ISSN: 1432-072X
    Keywords: Fumarate reductase ; Electron transport ; Cytochromes-Inhibition: HQNO, antimycin A ; Proteus mirabilis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1. Proteus mirabilis formed fumarate reductase under anaerobic growth conditions. The formation of this reductase was repressed under conditions of growth during which electron transport to oxygen or to nitrate is possible. In two of three tested chlorateresistant mutant strains of the wild type, fumarate reductase appeared to be affected. 2. Cytoplasmic membrane suspensions isolated from anaerobically grownP. mirabilis oxidized formate and NADH with oxygen and with fumarate, too. 3. Spectral investigation of the cytoplasmic membrane preparation revealed the presence of (probably at least two types of) cytochromeb, cytochromea 1 and cytochromed. Cytochromeb was reduced by NADH as well as by formate to approximately 80%. 4. 2-n-Heptyl-4-hydroxyquinoline-N-oxide and antimycin A inhibited oxidation of both formate and NADH by oxygen and fumarate. Both inhibitors increased the level of the formate/oxygen steady state and the formate/fumarate steady state. 5. The site of inhibition of the respiratory activity by both HQNO and antimycin A was located at the oxidation side of cytochromeb. 6. The effect of ultraviolet-irradiation of cytoplasmic membrane suspensions on oxidation/reduction phenomena suggested that the role of menaquinone is more exclusive in the formate/fumarate pathway than in the electron transport route to oxygen. 7. Finally, the conclusion has been drawn that the preferential route for electron transport from formate and from NADH to fumarate (and to oxygen) includes cytochromeb as a directly involved carrier. A hypothetical scheme for the electron transport in anaerobically grownP. mirabilis is presented.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 112 (1977), S. 25-34 
    ISSN: 1432-072X
    Keywords: Oxidative phosphorylation ; Proton translocation ; Continuous culture ; Sulphate limitation ; Iron limitation ; Growth yield ; Maintenance energy ; Paracoccus denitrificans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paracoccus denitrificans was aerobically grown in chemostat culture with succinate or gluconate as carbon source. Due to the presence of two phosphorylation sites in the respiratory chain and the absence of branching, theoretical P/O ratios of 1.71 and 1.82 were calculated for cells growing respectively with succinate and gluconate as carbon source. Using these data, 95% confidence intervals for the P/O ratio were determined, via a mathematical model, at 0.91–1.15 and 1.00–1.37 for sulphate-limited cultures, with respectively succinate and gluconate as carbon source. These results and measurements of P/O ratios in membrane particles and of proton translocation in whole cells have led to the conclusion that site I phosphorylation is affected under sulphate-limited conditions. Under conditions of carbon source-limitation the endogenous →H+/O ratio is about 7–8. Average values of 3.40 and 4.78 were respectively found for sulphate-limited succinate- and gluconate grown cells. For starved cells, oxidizing succinate as exogenous substrate, the →H+/O ratios were determined at about 3–4, independent of the growth limiting factor. It is concluded that the number of protons ejected per pair of electrons per energy-conserving site (→H+/site ratio) is about 3–4, instead of 2 as postulated by the chemiosmotic hypothesis.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 89 (1973), S. 327-339 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The efficiency of oxidative phosphorylation was estimated in intact “resting” cells of Proteus mirabilis, Escherichia coli B, Aerobacter aerogenes, Pseudomonas aeruginosa and Azotobacter vinelandii, measuring the change of ATP, ADP, AMP and NAD+ occurring on addition of limiting amounts of O2 to anaerobic cells of these bacteria. Also, in some cases O2-uptake was measured directly with a vibrating Pt-electrode. 2. Intact cells of P. mirabilis exhibited oxidative phosphorylation with a P/O-ratio of about 0.6–1.0. For A. aerogenes, P. aeruginosa and A. vinelandii a P/O-ratio of 0.30; 0.50 and 1.0 was found, respectively. 3. The reduction of NO3 - by anaerobic cells of P. mirabilis was accompanied by phosphorylation with a P/2e-(NO3 -)-ratio of about 0.37. 4. Changes in NAD+ were not representative of the amount of O2 consumed. So, methods in which the oxidation of NADH alone is taken as a measure for oxidative phosphorylation in bacteria do not give reliable P/2e--ratios. 5. It is concluded that P/O-ratios in intact “resting” bacterial cells are not as high as those for intact mitochondria from higher organisms and are also lower than P/O-ratios found for growing cells of bacteria.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Stochastic environmental research and risk assessment 6 (1992), S. 304-320 
    ISSN: 1436-3259
    Keywords: geostatistics ; precipitation ; water balance models ; semivariogram ; kriging ; spatial variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Daily precipitation amounts show spatial variation over sub-continential regions. Point measurements, represntative for regions of land, have to be interpolated towards unobserved locations. In this study four days in 1984 were selected to investigate the spatial variability of daily precipitation amount in north-western Europe in relation to the meteorological conditions. Data were interpolated using kriging. Crossvalidation was used to compare interpolated values with measured values. Large differences in the spatial structure of daily precipitation amount are observed as a result of different meteorological conditions. Stratification of the study area into a coast, a mountain and an interior stratum proved to be successful, reducing the Mean Squared Error of Prediction with up to 55%.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Stochastic environmental research and risk assessment 6 (1992), S. 209-221 
    ISSN: 1436-3259
    Keywords: geostatistics ; precipitation ; water balance models ; semivariogram ; kriging ; spatial variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract Daily precipitation amounts show spatial variation over sub-continential regions. Point measurements, representative for regions of land, have to be interpolated towards unobserved locations. In this study four days in 1984 were selected to investigate the spatial variability of daily precipitation amount in North-western Europe in relation to the meteorological conditions. Data were interpolated using Kriging. Crossvalidation was used to compare interpolated values with measured values. Large differences in the spatial structure of daily precipitation amount are obsered as a result of different meterological conditions. Stratification of the study area into a coastal, a mountainous and an interior stratum proved to be successful, reducing the Mean Squared Error of Prediction with up to 55%.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 43 (1975), S. 153-169 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Amongst the family of the Enterobacteriaceae the ability to reduce tetrathionate to thiosulfate and thiosulfate to sulfite and sulfide occurs in the genera Proteus, Citrobacter and Salmonella. These reductions are coupled to a respiratory chain which functions under anaerobic conditions. Only during transport of electrons to tetrathionate oxidative phosphorylation has been demonstrated. Isolation and purification of the cytoplasmic membrane bound tetrathionate and thiosulfate reductase fromProteus mirabilis makes clear that this bacterium forms only one enzyme for both reductions. This enzyme has a molecular weight of 133,000 daltons and can be divided into two subunits with molecular weights of 43,000 and 90,000 daltons by treatment with sodium dodecyl sulfate and 2-mercaptoethanol. The reduction of tetrathionate is activated by its primary product thiosulfate. Nitrate or oxygen represses and inactivates the tetrathionate and thiosulfate reductase. Nevertheless the smaller subunit of this enzyme appears to be formed and assembled into the cytoplasmic membranes after anaerobic growth in the presence of nitrate.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 43 (1975), S. 153-169 
    ISSN: 1573-5036
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Amongst the family of the Enterobacteriaceae the ability to reduce tetrathionate to thiosulfate and thiosulfate to sulfite and sulfide occurs in the genera Proteus, Citrobacter and Salmonella. These reductions are coupled to a respiratory chain which functions under anaerobic conditions. Only during transport of electrons to tetrathionate oxidative phosphorylation has been demonstrated. Isolation and purification of the cytoplasmic membrane bound tetrathionate and thiosulfate reductase fromProteus mirabilis makes clear that this bacterium forms only one enzyme for both reductions. This enzyme has a molecular weight of 133,000 daltons and can be divided into two subunits with molecular weights of 43,000 and 90,000 daltons by treatment with sodium dodecyl sulfate and 2-mercaptoethanol. The reduction of tetrathionate is activated by its primary product thiosulfate. Nitrate or oxygen represses and inactivates the tetrathionate and thiosulfate reductase. Nevertheless the smaller subunit of this enzyme appears to be formed and assembled into the cytoplasmic membranes after anaerobic growth in the presence of nitrate.
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  • 8
    Publication Date: 1992-09-01
    Print ISSN: 1436-3240
    Electronic ISSN: 1436-3259
    Topics: Architecture, Civil Engineering, Surveying , Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Published by Springer
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  • 9
    Publication Date: 1973-01-01
    Print ISSN: 0003-9276
    Topics: Biology
    Published by Springer
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  • 10
    Publication Date: 1977-02-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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