Publication Date:
2001-09-08
Description:
In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta-globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lykke-Andersen, J -- Shu, M D -- Steitz, J A -- CA 16038/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2001 Sep 7;293(5536):1836-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Molecular Biochemistry and Biophysics, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11546874" target="_blank"〉PubMed〈/a〉
Keywords:
3' Untranslated Regions/genetics/metabolism
;
Animals
;
Cell Line
;
DNA-Binding Proteins/genetics/*metabolism
;
Exons/*genetics
;
Fungal Proteins/metabolism
;
Globins/genetics
;
HeLa Cells
;
Humans
;
Macromolecular Substances
;
Mice
;
Models, Biological
;
Precipitin Tests
;
Protein Binding
;
RNA Helicases/metabolism
;
RNA Splicing
;
RNA, Messenger/genetics/*metabolism
;
RNA-Binding Proteins/genetics/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
*Ribonucleoproteins
;
Saccharomyces cerevisiae Proteins
;
Trans-Activators
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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