ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The aim of this study was to estimate the mechanical properties and evaluate thebiocompatibility of silk and PGA scaffolds as an artificial ligament to an ACL reconstruction. Thescaffold for the artificial ligament was braided / knitted silk or PGA thread. The mechanicalproperties, cell growth, and subcutaneous tissue reactions were determined for both types ofscaffolds. The breaking load of the PGA scaffold was double that of the sericin removed silkscaffold (SRSS). However, the initial attachment and growth of human ACL cells on the SRSS wassuperior to the PGA scaffold. In addition, the immune response was significantly higher on the PGAscaffold after 72 h (p〈0.05) compared with the sericin removed silk scaffold by T lymphocyte andmononuclear cells (MNCs) in vitro cultures. In vivo, the ACL scaffold made from silk or PGA wereimplanted in the subcutaneous layer in rats and harvested 1 week later. A histological evaluation ofthe scaffolds explants revealed the presence of monocytes in the SRSS, and an absence of giantcells in all cases. An inflammatory tissue reaction was more conspicuous around the silk scaffoldcontaining sericin and even more around the PGA scaffold compared with SRSS. These resultssupport the conclusion that a properly prepared SRSS, aside from providing benefits in terms ofbiocompatibility both in vitro and in vivo, can provide suitable scaffolds for the support of ACL cellgrowth. These results suggest that a SRSS for ACL repair can overcome the current limitations withthe PGA scaffold. And SRSS is biocompatible, and the in vitro T cell and MNCs culture modelshowed inflammatory responses that were comparable to those observed in vivo
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.73.pdf
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