ISSN:
0730-2312
Keywords:
glycopeptide
;
hepatocyte proliferation inhibitor
;
baby rat assay
;
α2-macroglobulin
;
high-performance liquid chromatography
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
A low molecular weight compound, which inhibits the G1-S transition in rat hepatocytes, was obtained by tryptic hydrolysis of human α2-macroglobulin followed by ultrafiltration at pH 10. It was purified by high-performance liquid chromatography on μBondapak C18 and μBondapak NH2 with a practically quantitative yield; from 5.1 g of α2-macroglobulin, 2.8 μg of purified compound were recovered. Inactivation by specific enzymes and chemical analyses showed that the inhibitor is a sialylated glycopeptide whose peptide moiety contains a pyroglutamyl residue. Its molecular mass, estimated by gel permeation chromatography, would be in the interval 3,500-4,600. However, amino acid analyses indicated that it is not yet pure. All these data suggest that α2-macroglobulin could be the carrier of the precursor form of the glycopeptide.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240400405
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