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  • 1
    Electronic Resource
    Electronic Resource
    Decrease in the mobility of proteins during electrophoresis in linear gradients of polyacrylamide (1986)
    Weinheim : Wiley-Blackwell
    Electrophoresis 7 (1986), S. 342-344 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Proteins with molecular weight between 66 000 and 950 000 were subjected to electrophoresis in 3 to 30 % linear gradients of polyacrylamide with volt-hour products between 2000 and 20 000. From the experimental data it was concluded that displacement of proteins decreased proportionally to the logarithm of time of electrophoresis. With these coordinates the fitness of the curves was found to be excellent (correlation coefficients were better than 0.993 for the analyzed proteins). These results support the hypothesis that there is no limit to be reached by a protein during its migration in a linear gradient of polyacrylamide. In addition we observed that the rate of decrease in mobility was proportional to the size of the protein.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150070711
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  • 2
    Electronic Resource
    Electronic Resource
    Multiple crossed immunoelectrophoresis on agarosecoated GelBond film (1985)
    Weinheim : Wiley-Blackwell
    Electrophoresis 6 (1985), S. 388-391 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A new technique, multiple crossed immunoelectrophoresis on agarose-coated GelBond film, is described. A sheet of GelBond with agarose areas containing a single or different antisera can be used for the simultaneous analysis of eight samples. Antigens were separated in the first dimension (i) in an inert medium according to net charge (cellulose acetate membrane elecrophoresis), (ii) in a sieving medium according to charge and molecular size (ultrathin-layer polyacrylamide gradient gel electrophoresis), or (iii) in a pH gradient according to differences in isoelectric points (ultrathinlayer isoelectric focusing). By using these methods in the first dimension, resolution is improved over that in conventional agarose electrophoresis. The first dimensional strips are easily transferred to the second dimensional antibody-containing agarose. Use of the GelBond film allows easy handling and convenient storage. Multiple crossed immunoelectrophoresis is a versatile method for rapid analysis of antigenic variability.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150060807
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    Paper (German National Licenses)
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