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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 64 (1993), S. 1797-1802 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Very sensitive laser light scattering equipment has been designed to be used in conjunction with chromatographic column separation. The system is prealigned and fully computer controlled. It gives the hydrodynamic radius of the eluting particles on line during separation. With a He-Ne laser source, the sensitivity is reached with four optical channels and four correlation functions of the scattered light measured in parallel with a special correlator.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 66 (1995), S. 2405-2410 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An integrated instrumentation which combines in the same system static and dynamic laser light scattering and time-resolved fluorescence spectroscopy is described. The two types of measurements can be performed simultaneously on the same sample contained in a thermostated cuvette. The light scattering allows measurements of molecular weights and hydrodynamic radii in the range of 20 A(ring) to a few micrometers. The overall time resolution of fluorescence measurements is around 50 ps. The system provides the simultaneous acquisition of the fluorescence decay curve, of the time integrated emission spectrum, and of two time-gated spectra. The light-scattering technique gives information on the global structure of the aggregates, while fluorescence measurements are sensitive to the local interactions of a fluorescence probe with the surroundings. The contemporary use of these two complementary techniques can be quite valuable mostly for biological samples for which stability and reproducibility with time can sometimes be a problem. As an example, combined measurements of the dimension of dimyristoylphosphatidylcholine vesicles around their temperature transition and of fluorescence lifetimes of the diphenylhexatriene probe incorporated in the vesicles is shown. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 65 (1994), S. 263-264 
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A simple digital correlator with four independent inputs is described. It gives 20 correlation points for each channel, with a maximum sampling frequency of 1 MHz, and is fully computer controlled. The correlator is designed to match the requirements of dynamic light scattering detectors in liquid chromatography.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biological cybernetics 61 (1989), S. 195-203 
    ISSN: 1432-0770
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Computer Science , Physics
    Notes: Abstract The use of autoregressive modelling has acquired great importance in time series analysis and in principle it may also be applicable in the spectral analysis of point processes with similar advantages over the nonparametric approach. Most of the methods used for autoregressive spectral analysis require positive semidefinite estimates for the covariance function, while current methods for the estimation of the covariance density function of a point process given a realization over the interval [0,T] do not guarantee a positive semidefinite estimate. This paper discusses methods for the estimation of the covariance density and conditional intensity function of point processes and present alternative computational efficient estimation algorithms leading always to positive semidefinite estimates, therefore adequate for autoregressive spectral analysis. Autoregressive spectral modelling of point processes from Yule-Walker type equations and Levinson recursion combined with the minimum AIC or CAT principle is illustrated with neurobiological data.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 15 (1977), S. 648-655 
    ISSN: 1741-0444
    Keywords: E.M.G. ; Spectral analysis ; Muscle ; Spike trains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire La génération d'un électromyogramme peut être mise en modèle par le filtrage linéaire des trains d'impulsionsN, considérés en tant qu'entrées neurales des unités motricesN, ou l'e.m.g. représente la somme des sorties des filtresN. En prenant ce modèle comme point de départ, on peut constater que non seulement les configurations des images potentielles de l'actionN, vues par les électrodes, représentent un facteur important pour l'établissement de la définition du spectre d'électromyogramme, mais que la distribution des intervalles entre-crête des trains d'impulsions le sont également. Similairement, on peut constater que les modifications d'ordre statistique des trains d'impulsions affectent l'extrémité de basse-fréquence du spectre de puissance, même si les potentiels actifs de l'unité motrice ne changent pas de configuration. Cet effet est illustré par l'utilisation de donées déjà publiées qui se rapportent aux aspects des muscles deltoides et des bicep brachiaux. Une méthode est également présentee, qui consiste à incorporer la synchronisation de l'unité motrice et, à cet égard, on constate que l'effet de cette dernière peut être possiblement à l'inverse de celle qui est engendrée par les statistiques des temps d'allumage.
    Abstract: Zusammenfassung Die Erzeugung eines EMG kann durch lineares Filtern einerN-Impulsserie im Modell dargestellt werden, die als neurale Eingaben fürN-motorische Einheiten angesehen werden, wobei das EMG die Summe der Ausgaben von denN-Filtern ist. Ausgehend von diesem Modell wird gezeigt, daß die Formen der durchN-Wirkung hervorgerufenen Potentialbilder, die von den Elektroden abgelesen werden, nicht nur einen bedeutenden Faktor in der Begrenzung des Spektrums darstellen, sondern daß dies auch für die Intervall- verteilung der Impulsserien zwischen den Spitzen zutrifft. Änderungen inder Statistik der Impulsserien wirken sich erwiesenermaßen auf die Nieder-frequenzseite des Kraftspektrums aus, selbst wenn die Aktionspotentiale der motorischen Einheiten ihre Form nicht ändern. Die Wirkung wird durch Einsatz von bereits veröffentlichten Daten über den Deltamuskel und die Brachialmuskeln dargestellt. Ein Mittel zum Einschluß der Synchronisation von motorischen Einheiten wird vorgestellt, und eswird gezeigt, daß seine Wirkung entegengesetzt zur Auslösungsstatistik liegen kann.
    Notes: Abstract The generation of an e.m.g. can be modelled by linear filtering of N impulse trains, regarded as the neural inputs to N motor units, where the e.m.g. is the sum of the outputs of the N filters. Starting from this model, it is shown that not only are the shapes of the N action potential images as viewed by the electrodes an important factor in defining the spectrum, but so is the interspike interval distribution of the impulse trains. Changes in the statistics of the pulse trains are shown to affect the low-frequency end of the power spectrum even if the motor-unit action potentials do not change shape. The effect is illustrated by use of data already published on deltoid muscle and brachii biceps. A means of including motor-unit synchronisation is introduced and it is shown that its effect can be contrary to that due to the firing statistics.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 19 (1981), S. 779-782 
    ISSN: 1741-0444
    Keywords: e.m.g. ; spectral analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 20 (1982), S. 649-652 
    ISSN: 1741-0444
    Keywords: E.M.G. analysis ; Microcomputer applications ; Spike analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 20 (1982), S. 44-48 
    ISSN: 1741-0444
    Keywords: Neurons ; Spectral analysis ; Spike trains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The links between the estimation of the spectral density of a train of δ functions, the estimation of the spectral density of a point process and that of a discrete random signal are established. It shows in particular that it is possible to reduce the estimation of the spectral density of a neural spike train to that of a regularly sampled 0–1 signal with consequent computational advantages. In addition, a technique involving decimation for speeding up the estimation of the spectral density without much additional error is proposed.
    Type of Medium: Electronic Resource
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  • 9
    Publication Date: 1995-03-01
    Print ISSN: 0034-6748
    Electronic ISSN: 1089-7623
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
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  • 10
    Publication Date: 2014-04-05
    Description: The incidence of inflammatory bowel disease is increasing worldwide and the underlying molecular mechanisms are far from being fully elucidated. Herein, we evaluated the role of N- glycosylation dysregulation in T cells as a key mechanism in the ulcerative colitis (UC) pathogenesis. The evaluation of the branched N -glycosylation levels and profile of intestinal T cell receptor (TCR) were assessed in colonic biopsies from UC patients and healthy controls. Expression alterations of the glycosyltransferase gene MGAT5 were also evaluated. We demonstrated that UC patients exhibit a dysregulation of TCR branched N -glycosylation on lamina propria T lymphocytes. Patients with severe UC showed the most pronounced defect on N -glycan branching in T cells. Moreover, UC patients showed a significant reduction of MGAT5 gene transcription in T lymphocytes. In this study, we disclose for the first time that a deficiency in branched N -glycosylation on TCR due to a reduced MGAT5 gene expression is a new molecular mechanism underlying UC pathogenesis, being a potential novel biomarker with promising clinical and therapeutic applications.
    Print ISSN: 0964-6906
    Electronic ISSN: 1460-2083
    Topics: Biology , Medicine
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