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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 7 (1975), S. 59-64 
    ISSN: 1432-1432
    Keywords: Fraction I Protein ; Ribulose Diphosphate Carboxylase ; Nicotiana ; Origin of Species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The polypeptide composition of Fraction I protein fromNicotiana digluta, a synthetic species which arose by chromosome doubling following the interspecific hybridization ofN.glutinosa andN.tabacum, has been examined by isoelectric focusing. The composition of the protein fromN.digluta, which was identical to the protein from the infertile F1 hybridN.glutinosa xN.tabacum, showed 3 polypeptides in the large subunit and 4 polypeptides in the small subunit. The large subunit polypeptides were identical to those fromN.glutinosa, the maternal parent in the original hybridization, whereas the small subunit polypeptides were a composite of the small subunit polypeptides from bothN.glutinosa andN.tabacum. This analysis demonstrates how the polypeptide composition of Fraction I protein evolves during the origin of new species ofNicotiana.
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 1172 (1993), S. 200-204 
    ISSN: 0167-4781
    Keywords: (Soybean) ; Protein kinase ; Structural analysis ; cDNA sequence
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Many abiotic environmental factors elicit the production of stress-ethylene in higher plants. To elucidate the molecular mechanisms underlying the regulation of stress-ethylene production and the physiological roles played by stress-ethylene in stress responses of plants, we studied the gene expression of ACC synthase in tobacco plants that had been subjected to environmental stresses. Four new tobacco ACC synthase cDNA fragments, NT-ACS2, NT-ACS3, NT-ACS4 and NT-ACS5, were identified and sequenced. It was found that NT-ACS2 could be induced by wounding, cold temperature and, especially, sunlight. NT-ACS4 was induced at a faster kinetics by wounding. The multiple environmental stress-responsive (MESR) NT-ACS2 gene was found to contain three introns and four exons and encode a polypeptide of 484 amino acids, 54·6 kDa and pI 6·87. Computer analysis of the 3·4 kb 5′ flanking region upstream of the ACS coding region revealed the existence of a group of putative cis-acting regulatory elements potentially conferring wounding, chilling, and UV light inducibility. Phylogenetic analysis of ACC synthase genes of different plant origins indicated that the chill-inducible NT-ACS2 gene is closely related to a chilling-inducible citrus ACS gene.
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 282 (1991), S. 98-102 
    ISSN: 0014-5793
    Keywords: Gene multiplicity ; Polymerase chain reaction ; Protein kinase ; Rice seedling ; Signal transduction
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 82 (1991), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: There are three prerequisites for the development of genetic tumors in Nicotiana; distantly related species must be crossed interspecifically, the hybrid plants must be exposed to stress conditions and an elevated level of cytokinin must be present. These events occur sequentially. First, the interspecific hybrid plants appear to produce a unique tumor-inducing factor (Ti-factor). Second, stress conditions trigger the synthesis signal molecule(s) which in turn stimulate synthesis of increased levels of cytokinin. Third, the elevated level of cytokinin acts upon the Ti-factor to initiate uncontrolled growth. The predominant shooty/leafy morphology of genetic tumors reflects the high level of endogenous cytokinin, and this hormone appears to play a pivotal role in the development of these tumors.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 43 (1978), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fraction 1 and unfractionated leaf proteins have been prepared from tobacco. Their amino acid compositions are very similar and have a high nutritional value. Removal of proteins from tobacco leaves before the curing process would result in a safer smoking product. Therefore, tobacco plants could be used for a rich food source and safer smoke material.
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  • 7
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 252 (1974), S. 226-227 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Polypeptide composition of Fraction I proteins from N. tabacum and the putative progenitor species. Crystalline Fraction I protein was dissolved in 8 M urea-0.5 M Tris-HCl, pH 8.5 and reduced by the addition of dithiothreitol. The protein was S-carboxymethylated by the addition of iodoacetic ...
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 259 (1976), S. 325-326 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fraction 1 protein consists of eight large and eight small subunits6. The large subunits are both encoded and syn-thesised within the chloroplast7'8 and seem to function as the catalytic sites for the carboxylase/oxygenase functions of this enzyme9. In contrast, the small subunits are both encoded ...
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 10 (1986), S. 819-822 
    ISSN: 1432-0983
    Keywords: Prokaryotic promoters ; Chloroplast DNA replication origin ; Chlamydomonas reinhardtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pK01 was used to screen for the presence of any prokaryotic promoter within the cloned fragment. The insertion of 2 AluI fragments yielded galK+ colonies. Sequence analyses of these Alul inserts revealed prokaryotic promoter consensus regions. Cloning into pKOTWI and subsequent DNA sequencing were used to determine the promoter-active orientation of each insert. Two back-to-back prokaryotic promoters were mapped on a 79 by Alul fragment located within the displacement loop region.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 60 (1981), S. 173-178 
    ISSN: 1432-2242
    Keywords: Chloroplast DNA ; RuBPCase ; Ribosomes ; Photosystems ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Under greenhouse conditions the dark green wild type (su/su) tobacco grows 2–3 times faster than the yellow mutant (Su/su) and contains five-fold more chlorophyll. On a fresh weight basis, however, both genotypes contain similar amounts of RuBPCase and fraction 11 protein in approximately equal proportion and have similar levels of 70s and 80s ribosomes. When seedlings are cultured on agar medium supplemented with sucrose and equal concentrations of IAA and kinetin or kinetin alone, a drastic reduction of RuBPCase and free 70s ribosomes, but not of chlorophyll content, were observed. Moreover, albino (Su/Su) seedlings developed on supplemented media still contain appreciable amounts of RuBPCase and free 70s ribosomes although chlorophyll levels are extremely low indicating no correlation between RuBPCase and chlorophyll content. RuBPCase crystallized from both wild type and yellow mutant plants seem to have identical composition and structure when examined by isoelectric focusing, amino acid analysis or peptide mapping techniques. The slow-growing yellow mutant is apparently deficient only in chlorophyll of the light harvesting chlorophyll-protein complex but with no alteration of the protein moiety or chlorophyll a/b ratio.
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