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  • 1
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    A localized Wnt signal orients asymmetric stem cell division in vitro (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2013-03-23
    Description: Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-beta-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear beta-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966430/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966430/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Habib, Shukry J -- Chen, Bi-Chang -- Tsai, Feng-Chiao -- Anastassiadis, Konstantinos -- Meyer, Tobias -- Betzig, Eric -- Nusse, Roel -- 102513/Wellcome Trust/United Kingdom -- GM063702/GM/NIGMS NIH HHS/ -- NS069375/NS/NINDS NIH HHS/ -- R01 GM030179/GM/NIGMS NIH HHS/ -- R01 GM063702/GM/NIGMS NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2013 Mar 22;339(6126):1445-8. doi: 10.1126/science.1231077.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Developmental Biology, Howard Hughes Medical Institute, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 265 Campus Drive, Stanford, CA 94305, USA. shabib@stanford.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23520113" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Asymmetric Cell Division ; *Cell Differentiation ; Cells, Cultured ; Centrosome/physiology ; Cytokinesis ; Embryonic Stem Cells/*cytology/*metabolism ; Gene Expression ; Homeodomain Proteins/genetics/metabolism ; Mice ; Mitosis ; Octamer Transcription Factor-3/genetics/metabolism ; Pluripotent Stem Cells/physiology ; Recombinant Proteins/metabolism ; Single-Cell Analysis ; Transcription Factors/genetics/metabolism ; Wnt Proteins/metabolism ; *Wnt Signaling Pathway ; Wnt3A Protein/*metabolism ; beta Catenin/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish (2010)
    National Academy of Sciences
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    Publication Date: 2010-11-01
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 3
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    Transposon-mediated BAC transgenesis in human ES cells (2012)
    Oxford University Press
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    Publication Date: 2012-10-24
    Description: Transgenesis is a cornerstone of molecular biology. The ability to integrate a specifically engineered piece of DNA into the genome of a living system is fundamental to our efforts to understand life and exploit its implications for medicine, nanotechnology and bioprospecting. However, transgenesis has been hampered by position effects and multi-copy integration problems, which are mainly due to the use of small, plasmid-based transgenes. Large transgenes based on native genomic regions cloned into bacterial artificial chromosomes (BACs) circumvent these problems but are prone to fragmentation. Herein, we report that contrary to widely held notions, large BAC-sized constructs do not prohibit transposition. We also report the first reliable method for BAC transgenesis in human embryonic stem cells (hESCs). The PiggyBac or Sleeping Beauty transposon inverted repeats were integrated into BAC vectors by recombineering, followed by co-lipofection with the corresponding transposase in hESCs to generate robust fluorescent protein reporter lines for OCT4, NANOG, GATA4 and PAX6. BAC transposition delivers several advantages, including increased frequencies of single-copy, full-length integration, which will be useful in all transgenic systems but especially in difficult venues like hESCs.
    Keywords: Synthetic Biology and Assembly Cloning, Recombination, DNA-Mediated Cell Transformation and Nucleic Acids Transfer
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 4
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    Concurrent Design Forces in Structures under Three-Component Orthotropic Seismic Excitation (2002)
    Sage Publications
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    Publication Date: 2002-02-01
    Description: According to the model of Penzien and Watabe, the three translational ground motion components on a specific point of the ground are statistically noncorrelated along a well-defined orthogonal system of axes p, w, and v, whose orientation remains reasonably stable over time during the strong motion phase of an earthquake. This orthotropic ground motion is described by three generally independent response spectra Sa, Sb, and Sc, respectively. The paper presents an antiseismic design procedure for structures according to the above seismic motion model. This design includes a) determination of the critical orientation of the seismic input, i.e., the orientation that gives the largest response, b) calculation of the maximum and the minimum values of any response quantity, and c) application of either the Extreme Stress Method or the Extreme Force Method for determining the most unfavorable combinations of several stress resultants (or sectional forces) acting concurrently at a specified section of a structural member.
    Print ISSN: 8755-2930
    Electronic ISSN: 1944-8201
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Published by Sage Publications on behalf of Earthquake Engineering Research Institute.
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  • 5
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    Equivalent Static Eccentricities in the Simplified Methods of Seismic Analysis of Buildings (1998)
    Sage Publications
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    Publication Date: 1998-02-01
    Description: The equivalent static eccentricities of seismic forces are usually defined by codes with simple expressions of the static eccentricity. This paper presents certain formulae for the exact calculation of these eccentricities on the basis of the dynamic response of a simplified model. From the parametric analysis of such formulae the determinative role of the torsional and lateral stiffness of the system becomes obvious for the correct evaluation of the equivalent static eccentricities. Finally, a proposal is made for the improvement of the static torsional provisions of the current codes.
    Print ISSN: 8755-2930
    Electronic ISSN: 1944-8201
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Published by Sage Publications on behalf of Earthquake Engineering Research Institute.
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