Publication Date:
1984-06-29
Description:
The chromosomal breakpoint of chronic lymphocytic leukemia (CLL) cells of the B-cell type carrying the translocated long arms of chromosomes 11 and 14 [t(11;14) (q13;q32)] was cloned. The breakpoint was found to be within the joining segment of the human heavy chain locus on the translocated long arm of chromosome 14. A probe that is specific for chromosome 11 and that maps immediately 5' to the breakpoint on the 14q+ chromosome was isolated. The probe detected a rearrangement of the homologous genomic DNA segment in the parental CLL cells and also in DNA from a diffuse large cell lymphoma with the t(11;14) translocation. This rearranged DNA segment was not present in Burkitt lymphoma cells with the t(8;14) translocation or in nonneoplastic human lymphoblastoid cells. The probe can thus be used to identify and characterize a gene located on band q13 of chromosome 11 that appears to be involved in the malignant transformation of human B cells carrying the t(11;14) translocation. This gene, named bcl -1, appears to be unrelated to any of the known retrovirus oncogenes described to date.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tsujimoto, Y -- Yunis, J -- Onorato-Showe, L -- Erikson, J -- Nowell, P C -- Croce, C M -- CA15882/CA/NCI NIH HHS/ -- CA16685/CA/NCI NIH HHS/ -- CA20034/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1984 Jun 29;224(4656):1403-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6610211" target="_blank"〉PubMed〈/a〉
Keywords:
Aged
;
Animals
;
B-Lymphocytes
;
Burkitt Lymphoma/genetics
;
Cell Line
;
*Chromosomes, Human, 13-15
;
*Cloning, Molecular
;
DNA, Neoplasm/genetics
;
DNA, Recombinant/metabolism
;
Humans
;
Hybrid Cells/metabolism
;
Leukemia/*genetics
;
Leukemia, Lymphoid/genetics
;
Lymphoma/*genetics
;
Male
;
Mice
;
Nucleic Acid Hybridization
;
*Translocation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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