Publication Date:
1992-07-10
Description:
Immature T cells and some T cell hybridomas undergo apoptotic cell death when activated through the T cell receptor complex, a phenomenon that is probably related to antigen induced negative selection of developing T cells. This activation-induced apoptosis depends on active protein and RNA synthesis in the dying cells, although none of the genes required for this process have previously been identified. Antisense oligonucleotides corresponding to c-myc block the constitutive expression of c-Myc protein in T cell hybridomas and interfere with all aspects of activation-induced apoptosis without affecting lymphokine production in these cells. These data indicate that c-myc expression is a necessary component of activation-induced apoptosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shi, Y -- Glynn, J M -- Guilbert, L J -- Cotter, T G -- Bissonnette, R P -- Green, D R -- AI31591/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1992 Jul 10;257(5067):212-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, University of Alberta, Edmonton, Canada.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1378649" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens, CD3
;
Antigens, Differentiation, T-Lymphocyte/physiology
;
Base Sequence
;
Blotting, Western
;
Cell Death/drug effects/genetics
;
Flow Cytometry
;
Gene Expression/drug effects
;
Genes, fos/physiology
;
Genes, myc/*physiology
;
Hybridomas
;
Lymphocyte Activation/drug effects/*physiology
;
Molecular Sequence Data
;
Oligonucleotides, Antisense/genetics/pharmacology
;
Proto-Oncogene Proteins c-myc/biosynthesis
;
RNA/biosynthesis
;
Receptors, Antigen, T-Cell/physiology
;
T-Lymphocytes/*physiology
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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