Publication Date:
2009-04-14
Description:
In microorganisms, noise in gene expression gives rise to cell-to-cell variability in protein concentrations. In mammalian cells, protein levels also vary and individual cells differ widely in their responsiveness to uniform physiological stimuli. In the case of apoptosis mediated by TRAIL (tumour necrosis factor (TNF)-related apoptosis-inducing ligand) it is common for some cells in a clonal population to die while others survive-a striking divergence in cell fate. Among cells that die, the time between TRAIL exposure and caspase activation is highly variable. Here we image sister cells expressing reporters of caspase activation and mitochondrial outer membrane permeabilization after exposure to TRAIL. We show that naturally occurring differences in the levels or states of proteins regulating receptor-mediated apoptosis are the primary causes of cell-to-cell variability in the timing and probability of death in human cell lines. Protein state is transmitted from mother to daughter, giving rise to transient heritability in fate, but protein synthesis promotes rapid divergence so that sister cells soon become no more similar to each other than pairs of cells chosen at random. Our results have implications for understanding 'fractional killing' of tumour cells after exposure to chemotherapy, and for variability in mammalian signal transduction in general.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858974/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858974/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Spencer, Sabrina L -- Gaudet, Suzanne -- Albeck, John G -- Burke, John M -- Sorger, Peter K -- CA112967/CA/NCI NIH HHS/ -- GM68762/GM/NIGMS NIH HHS/ -- P50 GM068762/GM/NIGMS NIH HHS/ -- P50 GM068762-06/GM/NIGMS NIH HHS/ -- U54 CA112967/CA/NCI NIH HHS/ -- U54 CA112967-05/CA/NCI NIH HHS/ -- England -- Nature. 2009 May 21;459(7245):428-32. doi: 10.1038/nature08012. Epub 2009 Apr 12.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Cell Decision Processes, Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19363473" target="_blank"〉PubMed〈/a〉
Keywords:
Apoptosis/*physiology
;
BH3 Interacting Domain Death Agonist Protein/metabolism
;
Caspases/metabolism
;
Cell Division
;
Cell Line
;
Enzyme Activation
;
Fluorescence Resonance Energy Transfer
;
Genes, Reporter
;
HeLa Cells
;
Humans
;
Mitochondrial Membranes/metabolism
;
Models, Biological
;
Permeability
;
Probability
;
Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism
;
Signal Transduction
;
TNF-Related Apoptosis-Inducing Ligand/*metabolism
;
Time Factors
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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