ISSN:
1432-203X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cells of the red alga Cyanidium caldarium were preincubated with 5 mmol 1−1 levulinic acid (LA) and subsequently incubated with 14C-labelled haem (5.67 Bq nmol−1). Phycocyanin was isolated. The specific radio-activity of its chromophore phycocyanobilin (PCB) was determined after cleavage and purification by thin layer chromatography. The percentage of PCB formed from labelled haem within 0.5 h was considerably higher in LA treated cells than in non-treated controls. This difference disappeared after prolonged incubation (16.5 h) with haem. The results are interpreted as possible incorporation of haem into preexisting apoprotein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00270172
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