Abstract
Cells of the red alga Cyanidium caldarium were preincubated with 5 mmol 1−1 levulinic acid (LA) and subsequently incubated with 14C-labelled haem (5.67 Bq nmol−1). Phycocyanin was isolated. The specific radio-activity of its chromophore phycocyanobilin (PCB) was determined after cleavage and purification by thin layer chromatography. The percentage of PCB formed from labelled haem within 0.5 h was considerably higher in LA treated cells than in non-treated controls. This difference disappeared after prolonged incubation (16.5 h) with haem. The results are interpreted as possible incorporation of haem into preexisting apoprotein.
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Abbreviations
- LA:
-
levulinic acid
- PCB:
-
phycocyanobilin
References
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Schuster, A., Köst, H.P., Rüdiger, W. et al. Incorporation of haem into phycocyanobilin in levulinic acid treated Cyanidium caldarium . Plant Cell Reports 2, 85–87 (1983). https://doi.org/10.1007/BF00270172
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DOI: https://doi.org/10.1007/BF00270172