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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 26 (1986), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The metabolism of the herbicide diclofop-methyl and the distribution of it and its metabolites have been investigated in a system utilizing isolated oat protoplasts (Avena sativa L. ‘Cascade’). Accumulations of 14C-diclofop-methyl were found in membrane fractions of the protoplasts. Diclofop-methyl partitioned into the lipid phase of the protoplast plasma membrane. Comparatively small amounts of diclofop were found associated with the protoplasts. Diclofop-methyl was converted to diclofop in the treatment solution by hydrolytic enzymes associated with the external surface of the protoplast. Approximately 78% of the radiolabel recovered from the treatment solution was diclofop. The two major accumulations of radiolabel were found in the water insoluble portion (membrane fraction) of the protoplast as diclofop-methyl and in the treatment solution as diclofop. Repartition du diclofop-methyl et de ses métabolites dans les protoplastes d'avoine (Avena sativa L.)Le métabolisme de l'herbicide diclofop-methyl (DM) et la répartition du DM et de ses métabolites ont étéétudiés dans un système faisant appel à des protoplastes d'avoine isolés (Avena sativa L.) ‘Cascade’. Des accumulations de 14C-diclofop-methyl ont été trouvées dans la fraction membranaire des protoplastes. Le diclofop-methyl est passé dans la phase pidique de la membrane plasmique du protoplaste. En comparaison, de petites quantités de diclofop ont été trouvées en association avec les protoplastes. Le diclofop-methyl a été converti en diclofop par des hydrolases associées à la surface externe des protoplastes. Approximativement 78% des traces radioactives retrouvées dans la solution de traitement étaient du diclofop. Les deux principales accumulations de radioactivitéétaient trouvées dans la fraction insoluble dans l'eau des protoplastes (fraction membranaire) sous forme de diclofop-méthyl et, dans la solution de traitement, sous forme de diclofop. Verteilung von Diclofop-methyl und Metaboliten in Hafer- (Avena sativa L.)-ProtoplastenDer Abbau von 14C-markiertem Diclofop-methyl und die Verteilung des Wirkstoffs sowie eines Metaboliten wurden in isolierten Protoplasten von Hafer (Avena sativa L. ‘Cascade’) untersucht. Der Wirkstoff war in den Protoplastenmembranen angereichert, wobei eine Anlagerung an die Lipidphase der Plasmamembran festgestellt wurde. Von Diclofop waren vergleichsweise geringe Mengen an die Protoplasten gebunden. Diclofop-methyl wurde in der Versuchslösung durch hydrolytische Enzyme, die an der Protoplastenaussenfläche lagen, zu Diclofop umgewandelt. Radioaktiv markierte Stoffe wurden hauptsächlich als Diclofop-methyl am Protoplasten-membransystem und als Diclofop (mit 78% der eingebrachten 14C-Aktivität) in der Lösung gefunden.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 24 (1975), S. 767-770 
    ISSN: 1573-5060
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The contribution of the host plant genome in symbiotic dinitrogen fixation has received little attention. In examining more than two thousand samples from the USDA Plant Introduction Collection, host-determined genetic variation in dinitrogen fixation has been found. In genetic analysis of a mutant line of Pisum resistant to nodulation, two genes designated Sym 2 and Sym 3 have been detected; Sym 2 affects nodulation while Sym 3 influences fixation. The two genes segregate independently as dominant Mendelian characters. Effective symbiosis requires the presence of at least one dominant gene at each locus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 32 (1983), S. 171-176 
    ISSN: 1573-5060
    Keywords: Pisum sativum ; field pea ; exogenous DNA ; legume nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The nodulation response of a non-nodulating field pea line (Pisum sativum L., PRL-H722) following treatment with DNA from an effectively nodulating cultivar (Trapper) has been investigated. DNA was introduced to the non-nodulating cultivar via imbibition and germination of seeds in DNA solutions under aseptic conditions. The DNA preparations used were 〉5×106 Daltons, had a smooth melting profile showing ≥40% hyperchromicity with a Tm=70–72°C in 0.1×SSC0 buffer, and 260/280 nm ratio of 〉1.8. A significant correction (ϰ2(I d.f.) = 2032**) of the mutant phenotype was observed in seeds treated with wild-type DNA. The nodulation response in treated plants was not observed in subsequent generations suggesting that the change observed did not involve integration of genetic information into the genome of germ line cells.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: cytokinin ; foliar spray ; kelp concentrates ; nitrogen nutrition ; Phaseolus vulgaris ; soil moisture stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse experiment was designed to test the effects of two kelp(Macrocystis integrifolia andEcklonia maxima) concentrates, when prepared as foliar sprays, upon bean (Phaseolus vulgaris) growth and N nutrition under three soil moisture regimes. Plant growth and developmental responses in this greenhouse experiment have demonstrated the effectiveness of the two kelp foliar sprays as plant growth regulating substances. Bean growth and developmental responses to the kelp foliar spray treatments were dependent upon the soil moisture regime to which they have been subjected. Although the two kelp foliar sprays had varying and sometimes contrasting effects on bean growth and N nutrition, which were dependent on the soil moisture treatment, their developmental effects upon the number of nodes, shoot/root ratio, leaf area ratio and specific leaf area were quite similar. A soybean callus bioassay demonstrated the presence of cytokinin-like substances and a callus growth antagonist in the kelp concentrate. Increasing dilution of the kelp concentrate disproportionately reduced the callus growth antagonist relative to the growth promoting or cytokinin-like activity.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 106 (1988), S. 281-284 
    ISSN: 1573-5036
    Keywords: plant growth promotion ; coadaptation ; Trifolium repens ; Lolium perenne ; Bacillus polymyxa ; genotypic specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of a plant growth promoting strain ofBacillus polymyxa was investigated using genotypically-defined mixtures of white clover (Trifolium repens L.) and perennial ryegrass (Lolium perenne L.). Addition ofB. polymyxa to a mixture of the species did not induce significant yield effects in perennial ryegrass, but resulted in a 23% (P〈0.05) yield increase in the clover component. The clover yield advantage increased further when clones of the legume were inoculated with theB. polymyxa genotypes with which they had previously coexisted in the field from which collections were made. The highest white clover yield was attained when clones of all three organisms (T. repens, L. perenne, andB. polymyxa) that had previously coexisted in the field were grown together in the experimental system.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 93 (1986), S. 287-291 
    ISSN: 1573-5036
    Keywords: Antiserum ; Intrinsic antibiotic resistance ; Rhizobium trifolii ; Strain identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Identification ofRhizobium trifolii strains using intrinsic antibiotic resistance and serology was performed. Unknown strains, generated by mixing known strains in a common broth, always reacted with only one antiserum but showed variable intrinsic antibiotic resistance patterns. All unknowns were rapidly and unambiguously identified by the immunological double diffusion technique, while 33% of the unknowns were either unidentifiable or identified incorrectly when resistance to various antibiotics was measured. It is concluded that serology is less variable than instrinsic antibiotic resistance when strains ofR. trifolii are identified.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 207 (1998), S. 147-154 
    ISSN: 1573-5036
    Keywords: inoculation ; rDNA ; phylogeny ; Rhizobium ; Trifolium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic relationships of Rhizobium isolated from temperate and tropical perennial Trifolium species were investigated using PCR-based nucleotide sequence analysis of 16S and 23S rDNA regions. Comparative analysis of partial 23S rDNA sequences clustered Rhizobium isolates effective with T. semipilosum, T. repens, T. pratense, T. hybridum and T. fragiferum into two distinct groups. These groups were consistent with the pattern of symbiotic effectiveness observed in cross-inoculation experiments. Our data suggested that strains from T. semipilosum were more closely related phylogenetically to R. etli, indicating that these strains do not belong in the R. leguminosarum bv trifolii group. Further differentiation of Rhizobium strains effective on T. semipilosum was reflected in the broader metabolic profile observed using the BIOLOG MicroPlate TM system to evaluate carbon utilization.
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  • 8
    Publication Date: 1978-08-01
    Print ISSN: 0018-067X
    Electronic ISSN: 1365-2540
    Topics: Biology
    Published by Springer Nature
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  • 9
    Publication Date: 1984-11-01
    Print ISSN: 0002-1962
    Electronic ISSN: 1435-0645
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 2000-05-01
    Description: Laboratory experiments were conducted with the millipede Harpaphe haydeniana haydeniana Wood (Polydesmida: Xystodesmidae) to determine (i) its litter feeding preferences, (ii) rates of leaf litter consumption, (iii) feeding effects on available nitrogen, and (iv) functional microbial diversity. The millipede exhibited a preference for Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and, to a lesser extent, Sitka spruce (Picea sitchensis (Bong.) Carrière) litter compared with western hemlock (Tsuga heterophylla (Raf.) Sarg.) and western redcedar (Thuja plicata Donn ex D. Don) litter when given a choice. When only one litter type was provided, millipedes consumed considerably more western redcedar than Douglas-fir, Sitka spruce, or western hemlock. Among the six broadleaf species tested, paper birch (Betula papyrifera Marsh.), bigleaf maple (Acer macrophyllum Pursh), vine maple (Acer circinatum Pursh), and red alder (Alnus rubra Bong.) were consumed at much higher rates than swordfern (Polystichum munitum (Kaulf.) Presl.) or salal (Gaultheria shallon Pursh). Daily rates of conifer litter consumption ranged between 10 and 20% of the millipede's fresh biomass and may translate to 36% of the annual litter fall. Our results suggest that transformation of conifer litter into millipede frass can increase rates of litter decomposition and N mineralization, as well as influence microbial activity and diversity in coastal forests.
    Print ISSN: 0045-5067
    Electronic ISSN: 1208-6037
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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