ISSN:
1573-0603
Keywords:
Cell immortalization
;
Rat embryo
;
Temperature sensitive gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe a method for prolonging the life span of primary cells using an N-terminal fragment of a temperature sensitive mutant of the polyoma virus oncogene, its large T antigen (LT). This allows long term characterization studies to be carried out on cells which would otherwise have senesced after a few passages in culture. Further, repeated preparation of cells from tissue sources can be avoided. Moreover, the cells can also be reverted to a ‘mortal’ state, if desired, by incubating at the non-permissive temperature for the LT function.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00986659
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