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  • 1
    facet.materialart.
    Unknown
    PANGAEA
    In:  Supplement to: Regaudie-de-Gioux, Aurore; Huete-Ortega, Maria; Sobrino, Cristina; López-Sandoval, Daffne C; González, N; Fernández-Carrera, Ana; Vidal, Montserrat; Marañón, Emilio; Cermeño, Pedro; Latasa, Mikel; Agustí, Susana; Duarte, Carlos Manuel (2019): Multi-model remote sensing assessment of primary production in the subtropical gyres. Journal of Marine Systems, 196, 97-106, https://doi.org/10.1016/j.jmarsys.2019.03.007
    Publication Date: 2023-09-20
    Description: Seawater was sampled from five depths in the euphotic zone corresponding to 100 % (ca. 3 m depth), 50 %, 20 %, 7 % and 1 % of incident Photosynthetically Active Radiation (PAR). For each depth, four 72 mL polystyrene bottles (three clear bottles and one dark bottle) were filled with unfiltered seawater, inoculated with 10 - 20 µCi NaH¹⁴CO₃ and incubated on-deck from dawn to dusk. Temperature and irradiance in the incubators simulated the water temperature and the incident irradiance at the corresponding depth of each sample by using a combination of neutral density and blue filters (Mist Blue, ref. 061, Lee Filters ®). After incubation, samples from three of the five depths (100 %, 20 % and 1 % PAR) were sequentially filtered through 20, 2 and 0.2 µm polycarbonate filters while the other depths (50 % and 7 % PAR) were directly filtered by 0.2 µm. Immediately after filtering, filters were then exposed to concentrated HCl fumes at least 12 h to remove the non-fixed inorganic ¹⁴C. Filters were placed in scintillation vials to which 5 mL of liquid scintillation cocktail was added. The radioactivity on each filter (disintegrations per minute, DPM) was determined using a Wallac scintillation counter. To compute the rate of photosynthetic carbon fixation, the dark-bottle DPM was subtracted from the light-bottle DPM values. A constant value of 24,720 µg L-1 (or 2,060 µmol L-1) was assumed for the concentration of dissolved inorganic carbon for surface waters in tropical ocean (Key et al., 2004). A correction factor of 1.05 was applied to this constant value for discrimination isotopic. Total primary production was calculated as the sum of the primary production on each size class.
    Keywords: 29HE20101215; 29HE20110117; 29HE20110211; 29HE20110317; 29HE20110416; 29HE20110513; 29HE20110619; CSIC; CTD/Rosette; CTD-RO; Date/Time of event; DEPTH, water; Event label; Hespérides; Latitude of event; Longitude of event; MALASPINA_LEG1; MALASPINA_LEG1_006-3; MALASPINA_LEG1_007-3; MALASPINA_LEG1_008-3; MALASPINA_LEG1_009-3; MALASPINA_LEG1_010-3; MALASPINA_LEG1_011-3; MALASPINA_LEG1_012-3; MALASPINA_LEG1_013-3; MALASPINA_LEG1_014-3; MALASPINA_LEG1_015-3; MALASPINA_LEG1_016-3; MALASPINA_LEG1_017-3; MALASPINA_LEG1_018-3; MALASPINA_LEG1_019-3; MALASPINA_LEG1_020-3; MALASPINA_LEG1_022-3; MALASPINA_LEG1_023-3; MALASPINA_LEG1_024-3; MALASPINA_LEG1_025-3; MALASPINA_LEG1_026-3; MALASPINA_LEG2; MALASPINA_LEG2_027-3; MALASPINA_LEG2_028-3; MALASPINA_LEG2_029-3; MALASPINA_LEG2_030-3; MALASPINA_LEG2_031-3; MALASPINA_LEG2_032-3; MALASPINA_LEG2_033-3; MALASPINA_LEG2_034-3; MALASPINA_LEG2_035-3; MALASPINA_LEG2_037-3; MALASPINA_LEG2_038-3; MALASPINA_LEG2_040-3; MALASPINA_LEG2_041-3; MALASPINA_LEG2_042-3; MALASPINA_LEG2_043-3; MALASPINA_LEG2_044-3; MALASPINA_LEG3; MALASPINA_LEG3_046-3; MALASPINA_LEG3_047-3; MALASPINA_LEG3_048-3; MALASPINA_LEG3_049-3; MALASPINA_LEG3_050-3; MALASPINA_LEG3_051-3; MALASPINA_LEG3_052-3; MALASPINA_LEG3_053-3; MALASPINA_LEG3_054-3; MALASPINA_LEG3_055-3; MALASPINA_LEG3_056-3; MALASPINA_LEG3_057-3; MALASPINA_LEG3_058-3; MALASPINA_LEG3_059-3; MALASPINA_LEG3_060-3; MALASPINA_LEG3_061-3; MALASPINA_LEG3_062-3; MALASPINA_LEG3_063-3; MALASPINA_LEG3_064-3; MALASPINA_LEG3_065-3; MALASPINA_LEG3_066-3; MALASPINA_LEG3_068-3; MALASPINA_LEG4; MALASPINA_LEG4_069-3; MALASPINA_LEG4_070-3; MALASPINA_LEG4_071-3; MALASPINA_LEG4_072-3; MALASPINA_LEG4_073-3; MALASPINA_LEG4_074-3; MALASPINA_LEG4_075-3; MALASPINA_LEG4_076-3; MALASPINA_LEG5; MALASPINA_LEG5_083-3; MALASPINA_LEG5_084-3; MALASPINA_LEG5_085-3; MALASPINA_LEG5_086-3; MALASPINA_LEG5_087-3; MALASPINA_LEG5_088-3; MALASPINA_LEG5_089-3; MALASPINA_LEG5_090-3; MALASPINA_LEG5_091-3; MALASPINA_LEG5_092-3; MALASPINA_LEG5_093-3; MALASPINA_LEG5_094-3; MALASPINA_LEG5_095-3; MALASPINA_LEG5_096-3; MALASPINA_LEG5_097-3; MALASPINA_LEG5_098-3; MALASPINA_LEG5_099-3; MALASPINA_LEG6; MALASPINA_LEG6_104-3; MALASPINA_LEG6_106-3; MALASPINA_LEG6_107-3; MALASPINA_LEG6_108-3; MALASPINA_LEG6_109-3; MALASPINA_LEG6_110-3; MALASPINA_LEG6_111-3; MALASPINA_LEG6_113-3; MALASPINA_LEG6_114-3; MALASPINA_LEG6_115-3; MALASPINA_LEG6_117-3; MALASPINA_LEG6_118-3; MALASPINA_LEG6_119-3; MALASPINA_LEG6_120-3; MALASPINA_LEG6_121-3; MALASPINA_LEG6_122-3; MALASPINA_LEG6_123-3; MALASPINA_LEG6_124-3; MALASPINA_LEG6_125-3; MALASPINA_LEG6_126-3; MALASPINA_LEG7; MALASPINA_LEG7_127-3; MALASPINA_LEG7_128-3; MALASPINA_LEG7_129-3; MALASPINA_LEG7_130-3; MALASPINA_LEG7_131-3; MALASPINA_LEG7_132-3; MALASPINA_LEG7_133-3; MALASPINA_LEG7_134-3; MALASPINA_LEG7_135-3; MALASPINA_LEG7_136-3; MALASPINA_LEG7_137-3; MALASPINA_LEG7_138-3; MALASPINA_LEG7_139-3; MALASPINA_LEG7_140-3; MALASPINA_LEG7_141-3; MALASPINA_LEG7_142-3; MALASPINA_LEG7_143-3; MALASPINA_LEG7_144-3; MALASPINA_LEG7_145-3; MALASPINA_LEG7_146-3; MALASPINA_LEG7_147-3; MALASPINA-2010; Malaspina circumnavigation expedition; MH008_006; MH009_007; MH010_008; MH011_009; MH012_010; MH013_011; MH014_012; MH015_013; MH016_014; MH017_015; MH018_016; MH019_017; MH020_018; MH021_019; MH022_020; MH024_022; MH025_023; MH026_024; MH027_025; MH028_026; MH036_027; MH037_028; MH038_029; MH039_030; MH040_031; MH041_032; MH042_033; MH043_034; MH044_035; MH046_037; MH047_038; MH049_040; MH050_041; MH051_042; MH052_043; MH053_044; MH062_046; MH063_047; MH064_048; MH065_049; MH066_050; MH067_051; MH072_052; MH073_053; MH074_054; MH075_055; MH076_056; MH077_057; MH078_058; MH079_059; MH080_060; MH081_061; MH082_062; MH083_063; MH084_064; MH085_065; MH086_066; MH088_068; MH095_069; MH096_070; MH097_071; MH098_072; MH099_073; MH100_074; MH101_075; MH102_076; MH127_083; MH128_084; MH129_085; MH130_086; MH131_087; MH132_088; MH133_089; MH134_090; MH135_091; MH136_092; MH137_093; MH138_094; MH139_095; MH140_096; MH141_097; MH142_098; MH143_099; MH153_104; MH155_106; MH156_107; MH157_108; MH158_109; MH159_110; MH160_111; MH162_113; MH163_114; MH164_115; MH166_117; MH167_118; MH168_119; MH169_120; MH170_121; MH171_122; MH172_123; MH173_124; MH174_125; MH175_126; MH188_127; MH189_128; MH190_129; MH191_130; MH193_131; MH194_132; MH195_133; MH196_134; MH197_135; MH198_136; MH199_137; MH200_138; MH201_139; MH202_140; MH203_141; MH204_142; MH205_143; MH206_144; MH207_145; MH208_146; MH209_147; primary production; Primary production of carbon, standard deviation; Primary production of carbon per hour; see abstract; subtropical gyres
    Type: Dataset
    Format: text/tab-separated-values, 1241 data points
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  • 2
    Publication Date: 2024-02-02
    Description: Biological nitrogen fixation is a key process balancing the loss of combined nitrogen in the marine nitrogen cycle. Its relevance in upwelling systems is not fully resolved. This dataset contains rates of nitrogen fixation through the euphotic layer in two size fractions measured following Montoya et al (1996) technique. It also contains the stable isotopes of carbon in seston expressed in delta notation (δ13C, ‰, VPDB). We sampled in the region of the Guinea Dome and Equatorial Atlantic Ocean along 23°W during Meteor cruise M130 in August-September 2016. Water samples were collected by niskin bottles attached to a rosette equipped with CTD sensors. Incubations were done in on-deck incubators refrigerated by running surface water continuously and simulating the light intensity of each depth by neutral density filters or meshes.
    Keywords: Abbreviation; biological nitrogen fixation; Calculated according to Fry (2006); Calculated according to Montoya et al. (1996); Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Isoprime; coupled with Elemental analyser, Carlo Erba, NA 2500; CTD, Sea-Bird, SBE 11 plus; CTD/Rosette; CTD-RO; DATE/TIME; DEPTH, water; diazotrophs; ELEVATION; Equatorial Atlantic; Event label; Gear; Guinea Dome; Incubation duration; LATITUDE; Location; LONGITUDE; M130; M130_1002-1; M130_1005-1; M130_1010-1; M130_1016-1; M130_1019-1; M130_1027-1; M130_1031-1; M130_1037-1; M130_1042-1; M130_1045-1; M130_1047-1; M130_1050-1; M130_1051-1; M130_1054-1; M130_1056-1; M130_1060-1; M130_1068-1; M130_1069-1; M130_1078-1; M130_1080-1; M130_1090-1; M130_1092-1; M130_1094-1; M130_1096-1; M130_1100-1; M130_1104-1; M130_941-1; M130_949-1; M130_952-1; M130_955-1; M130_957-1; M130_959-1; M130_962-1; M130_966-1; M130_969-1; M130_972-1; M130_974-1; M130_976-1; M130_981-1; M130_983-1; M130_989-1; M130_994-1; M130_998-1; Meteor (1986); Nitrogen; Nitrogen, 15N labeled; Nitrogen, particulate, size fraction 〈10 µm; Nitrogen, particulate, size fraction 〈10 µm, standard deviation; Nitrogen, particulate, size fraction 〉 10 µm; Nitrogen, particulate, size fraction 〉 10 µm, standard deviation; Nitrogen fixation rate, size fraction 〈 10 µm; Nitrogen fixation rate, size fraction 〈 10 µm, standard deviation; Nitrogen fixation rate, size fraction 〉 10 µm; Nitrogen fixation rate, size fraction 〉 10 µm, standard deviation; Nitrogen fixation rate, total; Nitrogen fixation rate, total, standard deviation; Salinity; Sample volume; Temperature, water; Trichodesmium, carbon in seston; δ13C, seston
    Type: Dataset
    Format: text/tab-separated-values, 1101 data points
    Location Call Number Expected Availability
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  • 3
    Publication Date: 2024-02-02
    Description: Biological nitrogen fixation is a key process balancing the loss of combined nitrogen in the marine nitrogen cycle. Its relevance in upwelling systems is not fully resolved. This dataset contains rates of nitrogen fixation through the euphotic layer in two size fractions measured following Montoya et al (1996) technique. It also contains the stable isotopes of carbon in seston expressed in delta notation (δ13C, ‰, VPDB). We sampled in the region of the Guinea Dome and Equatorial Atlantic Ocean along 23°W during Meteor cruise M119 in September 2015. Water samples were collected by niskin bottles attached to a rosette equipped with CTD sensors. Incubations were done in on-deck incubators refrigerated by running surface water continuously and simulating the light intensity of each depth by neutral density filters or meshes.
    Keywords: Abbreviation; biological nitrogen fixation; Calculated according to Fry (2006); Calculated according to Montoya et al. (1996); Continuous flow isotope ratio mass spectrometer (CF-IRMS), Micromass, Optima; coupled with Elemental analyser, Carlo Erba, NC2500; CTD, Sea-Bird, SBE 11 plus; CTD/Rosette; CTD003; CTD005; CTD007; CTD009; CTD010; CTD012; CTD013; CTD015; CTD018; CTD019; CTD022; CTD024; CTD026; CTD031; CTD032; CTD033; CTD035; CTD036; CTD038; CTD040; CTD042; CTD043; CTD045; CTD047; CTD049; CTD050; CTD051; CTD052; CTD053; CTD-RO; DATE/TIME; DEPTH, water; diazotrophs; ELEVATION; Equatorial Atlantic; Event label; Gear; Guinea Dome; Incubation duration; LATITUDE; Location; LONGITUDE; M119; M119_692-1; M119_698-1; M119_703-1; M119_706-1; M119_708-1; M119_718-1; M119_722-1; M119_724-1; M119_731-1; M119_733-1; M119_737-1; M119_740-1; M119_745-1; M119_755-1; M119_756-1; M119_758-1; M119_761-1; M119_762-1; M119_765-1; M119_770-1; M119_780-1; M119_781-1; M119_783-1; M119_785-1; M119_788-1; M119_789-1; M119_791-1; M119_792-1; M119_793-1; Meteor (1986); Nitrogen; Nitrogen, 15N labeled; Nitrogen, particulate, size fraction 〈10 µm; Nitrogen, particulate, size fraction 〈10 µm, standard deviation; Nitrogen, particulate, size fraction 〉 10 µm; Nitrogen, particulate, size fraction 〉 10 µm, standard deviation; Nitrogen fixation rate, size fraction 〈 10 µm; Nitrogen fixation rate, size fraction 〈 10 µm, standard deviation; Nitrogen fixation rate, size fraction 〉 10 µm; Nitrogen fixation rate, size fraction 〉 10 µm, standard deviation; Nitrogen fixation rate, total; Nitrogen fixation rate, total, standard deviation; Salinity; Sample code/label; Sample volume; Temperature, water; Trichodesmium, carbon in seston; δ13C, seston
    Type: Dataset
    Format: text/tab-separated-values, 1170 data points
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  • 4
    Publication Date: 2019-07-01
    Description: The 15N2-tracer assay [Montoya et al. (1996) A simple, high-precision, high-sensitivity tracer assay for N2 fixation. Appl. Environ. Microbiol., 62, 986–993.] is the most used method for measuring biological N2 fixation in terrestrial and aquatic environments. The reliability of this technique depends on the purity of the commercial 15N2 gas stocks used. However, Dabundo et al. [(2014) PLoS One, 9, e110335.] reported the contamination of some of these stocks with labile 15N-labeled compounds (ammonium, nitrate and/or nitrite). The contamination of commercial 15N2 gas stocks with 15N-labeled nitrate and 142 ammonium and consequences for nitrogen fixation measurements. Considering that the tracer assay relies on the conversion of isotopically labeled 15N2 into organic nitrogen, this contamination may have led to overestimated N2 fixation rates. We conducted laboratory and field experiments in order to (i) test the susceptibility of 15N contaminants to assimilation by non-diazotroph organisms and (ii) determine the potential overestimation of the N2 fixation rates estimated in the field. Our findings indicate that the contaminant 15N-compounds are assimilated by non-diazotrophs organisms, leading to an overestimation of N2 fixation rates in the field up to 16-fold under hydrographic conditions of winter mixing.
    Print ISSN: 0142-7873
    Electronic ISSN: 1464-3774
    Topics: Biology
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  • 5
  • 6
    Publication Date: 2019-01-08
    Print ISSN: 1539-607X
    Electronic ISSN: 1539-6088
    Topics: Biology , Geosciences
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  • 7
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  • 9
  • 10
    Publication Date: 2024-02-07
    Description: In the Equatorial Atlantic nitrogen availability is assumed to control phytoplankton dynamics. However, in situ measurements of phytoplankton physiology and productivity are surprisingly sparse in comparison with the North Atlantic. In addition to the formation of the Equatorial cold tongue in the boreal summer, tropical instability waves (TIWs) and related short-term processes may locally cause episodic events of enhanced nutrient supply to the euphotic layer. Here, we assess changes in phytoplankton photophysiology in response to such episodic events as well as short-term nutrient addition experiments using a pair of custom-built fluorometers that measure chlorophyll a (Chl a) variable fluorescence and fluorescence lifetimes. The fluorometers were deployed during a transatlantic cruise along the Equator in the fall of 2019. We hypothesized that the Equatorial Atlantic is nitrogen-limited, with an increasing degree of limitation to the west where the cold tongue is not prominent, and that infrequent nitrate injection by TIW related processes are the primary source alleviating this limitation. We further hypothesized phytoplankton are well acclimated to the low levels of nitrogen, and once nitrogen is supplied, they can rapidly utilize it to stimulate growth and productivity. Across three TIW events encountered, we observed increased productivity and chlorophyll a concentration concurrent with a decreased photochemical conversion efficiency and overall photophysiological competency. Moreover, the observed decrease in photosynthetic turnover rates toward the western section suggested a 70% decrease in growth rates compared to their maximum values under nutrient-replete conditions. This decrease aligned with the increased growth rates observed following 24 h incubation with added nitrate in the western section. These results support our hypotheses that nitrogen is the limiting factor in the region and that phytoplankton are in a state of balanced growth, waiting to “body surf” waves of nutrients which fuel growth and productivity.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
    Format: text
    Format: text
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