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  • 1
    Publication Date: 2021-05-19
    Description: This study was aimed to gain knowledge on the ontogeny of digestive enzymes in common carp larvae at the governmental Warm water Fish Aquaculture Center of Shahid Rajaee in Sari, Mazandaran, Iran. The ontogenetic development of pancreatic (trypsin, chymotrypsin, lipase and α-amylase) and intestinal (alkaline phosphatase and aminopeptidase-N) enzymes were assessed in common carp larvae from first feeding (3 days post hatching) to 33 days post hatching (dph). The larvae started to feed on rotifers at day 3 to day 7. Feeding on a commercial diet was started from day-8 onwards. Specific trypsin and chymotrypsin activities feed with rotifer were 0.011 U/mg protein and 0.003 U/mg protein at day 7, respectively then the specific activities feed with dry food reached a maximum level at 33 dph (0.028 U/mg protein and 0.028 U/mg protein, respectively) (P〈0.05) compared to live prey. The elevated alkaline proteases activity can be related to adaptation of larvae to digest protein content in the food. Specific lipase activity was 0.0006 U/mg protein at 7 dph then the activity feed with dry food reached a maximum level at 15 dph (0.0011 U/mg protein) (P〈0.05) compared to live prey. Specific α-amylase activity feed with rotifer was 85.86 U/mg protein on the 7 dph consequently, its specific activity reached a maximum level at 15 dph (128.77 U/mg protein) (P〈0.05). Specific alkaline phosphatase and aminopeptidase N activities feed with rotifer were 6 U/mg protein and 0.0106 U/mg protein at day 7, respectively then the specific activities feed with dry food reached a maximum level at 33 dph (28.66 U/mg protein and 0.58 U/mg protein, respectively) (P〈0.05) compared to live prey.
    Description: Published
    Keywords: Aquaculture ; Cyprinus carpio ; Common carp ; Pancreatic enzymes ; Larval growth ; Digestive enzymes ; Ontogeny ; Enzymes
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.320-334
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  • 2
    Publication Date: 2021-05-19
    Description: Effects of dietary supplementation of red macroalgae Gracilaria pygmea on the growth performance, hematology and serum biochemistry parameters of Asian Seabass Lates calcarifer with initial fish weight of 28±5.9 g were investigated in a 6-week experiment. The experimental diets were composed of the positive control diet (containing fishmeal), the negative control diet (containing fishmeal-soy protein) and three diets which supplemented with varying leves of red algae (3, 6 and 9%). Results indicated that daily growth rate, feed conversion ratio and final weight of fish not only were not negatively affected by different substitution levels of red algae, but also the final weight of fish fed the diet containing 6% red algae was higher than the other experimental diets. Condition factor of the negative control group and the specific growth rate of the treatment containing 6% red algae were significantly higher than those of the other experimental diets (P〈0.05). Results of hemoglobin, hematocrit, White Blood Cell (WBC) count and WBC differential count were not significantly different among various treatments (P〉0.05). Among the examined serum biochemistry parameters of Asian Seabass, only glucose was not significantly affected by various dietary treatments (P〉0.05). The cholesterol levels of the negative control group and fish fed the diet containing 9% red algae were similar with each other and were significantly higher than the other treatments (P〈0.05). The lowest level of albumin was observed in the treatment containing 6% red algae, whearese the lowest amounts of total protein, triglyceride and cholesterol were observed in the treatment containing 3% red algae. Overall, it seems that partial substitution of expensive fishmeal by red algae Gracilaria pygmaea in the diet of carnivorous Asian Seabass exerted no negative effects on the growth and immunity parameters. Moreover, some growth parameters, hematological and serum biochemical parameters of blood were improved by the diet containing 6% red algae.
    Description: Published
    Keywords: Gracilaria pygmaea ; Lates calcarifer ; Asian Seabass Lates calcarifer ; Gracilaria algae ; Diet ; Specific growth rate ; Innate immunity ; Substitution ; Growth performance ; Algae ; Biochemistry ; Serum ; Hematology
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.77-89
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/22601 | 18721 | 2018-05-01 22:51:33 | 22601 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-08
    Description: This study was aimed to gain knowledge on the ontogeny of digestive enzymes in common carp larvae at the governmental Warm Water Fish Aquaculture Center of Shahid Rajaee in Sari, Mazandaran, Iran. The ontogenetic development of pancreatic (trypsin, chymotrypsin, lipase and α-amylase) and intestinal (alkaline phosphatase and aminopeptidase-N) enzymes were assessed in common carp larvae from first feeding (3 days post hatching) to 33 days post hatching (dph). The larvae started to feed on rotifers at day 3 to day 7. Feeding on a commercial diet was started from day-8 onwards. Specific trypsin and chymotrypsin activities feed with rotifer were 0.011 U/mg protein and 0.003 U/mg protein at day 7, respectively; then the specific activities feed with dry food reached a maximum level at 33 dph (0.028 U/mg protein and 0.028 U/mg protein, respectively) (P〈0.05) compared to live prey. The elevated alkaline proteases activity can be related to adaptation of larvae to digest protein content in the food. Specific lipase activity was 0.0006 U/mg protein at 7 dph; then the activity feed with dry food reached a maximum level at 15 dph (0.0011 U/mg protein) (P〈0.05) compared to live prey. Specific α-amylase activity feed with rotifer was 85.86 U/mg protein on the 7 dph; consequently, its specific activity reached a maximum level at 15 dph (128.77 U/mg protein) (P〈0.05). Specific alkaline phosphatase and aminopeptidase N activities feed with rotifer were 6 U/mg protein and 0.0106 U/mg protein at day 7, respectively; then the specific activities feed with dry food reached a maximum level at 33 dph (28.66 U/mg protein and 0.58 U/mg protein, respectively) (P〈0.05) compared to live prey.
    Keywords: Aquaculture ; Biology ; Fisheries ; Common carp ; Pancreatic enzymes ; Larval growth ; Digestive enzymes ; Ontogeny ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 320-334
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