Publication Date:
2013-03-09
Description:
Cell-cell fusion is critical for the conception, development, and physiology of multicellular organisms. Although cellular fusogenic proteins and the actin cytoskeleton are implicated in cell-cell fusion, it remains unclear whether and how they coordinate to promote plasma membrane fusion. We reconstituted a high-efficiency, inducible cell fusion culture system in the normally nonfusing Drosophila S2R+ cells. Both fusogenic proteins and actin cytoskeletal rearrangements were necessary for cell fusion, and in combination they were sufficient to impart fusion competence. Localized actin polymerization triggered by specific cell-cell or cell-matrix adhesion molecules propelled invasive cell membrane protrusions, which in turn promoted fusogenic protein engagement and plasma membrane fusion. This de novo cell fusion culture system reveals a general role for actin-propelled invasive membrane protrusions in driving fusogenic protein engagement during cell-cell fusion.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3631436/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3631436/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shilagardi, Khurts -- Li, Shuo -- Luo, Fengbao -- Marikar, Faiz -- Duan, Rui -- Jin, Peng -- Kim, Ji Hoon -- Murnen, Katherine -- Chen, Elizabeth H -- R01 GM098816/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Apr 19;340(6130):359-63. doi: 10.1126/science.1234781. Epub 2013 Mar 7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23470732" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/*metabolism
;
Animals
;
Caenorhabditis elegans Proteins/genetics/*metabolism
;
Cell Adhesion Molecules/genetics/*metabolism
;
*Cell Communication
;
Cell Culture Techniques
;
*Cell Fusion
;
Cell Line
;
Cell Surface Extensions/metabolism/physiology
;
Drosophila Proteins/genetics/metabolism
;
Drosophila melanogaster/cytology
;
Immunoglobulins/genetics/metabolism
;
Membrane Glycoproteins/genetics/*metabolism
;
Membrane Proteins/genetics/metabolism
;
Muscle Proteins/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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